| RRC ID |
4851
|
| 著者 |
Koyano R, Ishida S, Fujiwara S.
|
| タイトル |
Transcriptional regulation of the retinoic acid receptor in the dorsal midline epidermis in the Ciona intestinalis embryo.
|
| ジャーナル |
Dev Growth Differ
|
| Abstract |
Retinoic acid regulates the spatial pattern of gene expression mainly in the epidermis in the protochordate ascidian, Ciona intestinalis. Our previous study characterized the enhancer element responsible for the activation of the retinoic acid receptor (RAR) in the dorsal midline epidermis (DME). In the present study, detailed analysis identified two small sequence elements required for the transcriptional activation in the DME. Deletion of either one of these sequences resulted in suppression of the DME enhancer, suggesting that both sites are necessary. The nucleotide sequences of these two sites were similar to the consensus recognition sequences for the Msx and Sox transcription factors, respectively. These transcription factors are expressed in the DME lineage blastomeres (b7.9 and b7.10 blastomere pairs of bilaterally symmetrical embryos of this species) at the 64-cell stage. Electrophoretic mobility shift assays revealed that recombinant Msxb and SoxB1 proteins specifically bound to these sequences. These results suggest that Msxb and SoxB1 synergistically activate the enhancer in the DME.
|
| 巻・号 |
51(9)
|
| ページ |
777-86
|
| 公開日 |
2009-12-1
|
| DOI |
10.1111/j.1440-169X.2009.01136.x
|
| PII |
DGD1136
|
| PMID |
19824898
|
| MeSH |
Animals
Animals, Genetically Modified
Base Sequence
Ciona intestinalis / embryology*
Ciona intestinalis / genetics*
Cloning, Molecular
Embryo, Nonmammalian
Epidermis / embryology
Epidermis / metabolism*
Gene Expression Regulation, Developmental
Homeodomain Proteins / metabolism
Homeodomain Proteins / physiology
Molecular Sequence Data
Receptors, Retinoic Acid / genetics*
Receptors, Retinoic Acid / metabolism
Regulatory Sequences, Nucleic Acid
SOXB1 Transcription Factors / metabolism
SOXB1 Transcription Factors / physiology
Sequence Homology, Nucleic Acid
Transcriptional Activation*
|
| IF |
1.723
|
| 引用数 |
0
|
|
WOS 分野
|
CELL BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| カタユウレイボヤ・(ニッポンウミシダ) |
Wild C.int |
