RRC ID 6836
著者 Ogasawara S, Shimada N, Kawata T.
タイトル Role of an expansin-like molecule in Dictyostelium morphogenesis and regulation of its gene expression by the signal transducer and activator of transcription protein Dd-STATa.
ジャーナル Dev Growth Differ
Abstract Expansins are proteins involved in plant morphogenesis, exerting their effects on cellulose to extend cell walls. Dictyostelium is an organism that possesses expansin-like molecules, but their functions are not known. In this study, we analyzed the expL7 (expansin-like 7) gene, which has been identified as a putative target of Dd-STATa, a Dictyostelium homolog of the metazoan signal transducer and activator of transcription (STAT) proteins. Promoter fragments of the expL7 were fused to a lacZ reporter and the expression patterns determined. As expected from the behavior of the endogenous expL7 gene, the expL7/lacZ fusion gene was downregulated in Dd-STATa null slugs. In the parental strain, the expL7 promoter was activated in the anterior tip region. Mutational analysis of the promoter identified a sequence that was necessary for expression in tip cells. In addition, an activator sequence for pstAB cells was identified. These sequences act in combination with the repressor region to prevent ectopic expL7 expression in the prespore and prestalk regions of the slug and culminant. Although the expL7 null mutant showed no phenotypic change, the expL7 overexpressor showed aberrant stalk formation. These results indicate that the expansin-like molecule is important for morphogenesis in Dictyostelium.
巻・号 51(2)
ページ 109-22
公開日 2009-2-1
DOI 10.1111/j.1440-169X.2009.01086.x
PII DGD1086
PMID 19207182
MeSH Amino Acid Sequence Animals Consensus Sequence Dictyostelium / genetics Dictyostelium / growth & development Dictyostelium / physiology* Gene Expression Regulation, Developmental* Genes, Protozoan Genes, Reporter Molecular Sequence Data Morphogenesis / genetics Morphogenesis / physiology Multigene Family Open Reading Frames Phenotype Phylogeny Plant Proteins / chemistry Plant Proteins / genetics Point Mutation Promoter Regions, Genetic / genetics Protozoan Proteins / genetics Protozoan Proteins / physiology* Recombinant Fusion Proteins / biosynthesis STAT Transcription Factors / deficiency STAT Transcription Factors / genetics STAT Transcription Factors / physiology* Sequence Alignment Sequence Homology, Amino Acid Species Specificity Transcription, Genetic
IF 1.723
引用数 17
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
細胞性粘菌 G01679