RRC ID 43401
著者 Sun C, Wu Z, Jia F, Wang Y, Li T, Zhao M.
タイトル Identification of zebrafish LPTS: a gene with similarities to human LPTS/PinX1 that inhibits telomerase activity.
ジャーナル Gene
Abstract Human LPTS/PinX1 is a newly identified telomerase inhibitory protein. Overexpression of the LPTS/PinX1 gene suppresses telomerase activity, results in shortened telomeres. To investigate the role of the LPTS gene in zebrafish, we cloned the homologous gene, zLPTS, which encodes a protein of 355 amino acids. Sequence analysis revealed that, like human LPTS/PinX1, the zLPTS protein has a conserved G-patch domain at its N-terminus and a lysine-rich domain at its C-terminus. Bioinformatics analysis showed the evolutionary conservation of zLPTS. Using RT-PCR and northern blot, we found that zLPTS was expressed in all zebrafish tissues with higher level in ovary, and in all embryonic developmental stages examined. Whole mount in situ hybridization revealed that zLPTS was expressed in all regions of early developmental embryos. The subcellular localization of zLPTS protein was showed in the nucleolus and telomeres. We also cloned the gene for zebrafish Telomerase Reverse Transcriptase (zTERT), a catalytic subunit of telomerase, and demonstrated that zLPTS protein can interact with zTERT through the TR-binding domain of zTERT. Further, we verified that zLPTS could inhibit telomerase activities in zebrafish embryos and human cancer cell line by TRAP assay. Our results clearly demonstrate that zLPTS is ubiquitously expressed in tissues and embryos and plays a function of inhibiting telomerase activity. This study may provide a useful system for further investigating the mechanism of telomere length regulation.
巻・号 420(1)
ページ 90-8
公開日 2008-8-15
DOI 10.1016/j.gene.2008.05.013
PII S0378-1119(08)00209-6
PMID 18583067
MeSH Amino Acid Sequence Animals Cell Cycle Proteins Female Gene Expression Profiling Humans Molecular Sequence Data Nuclear Proteins / genetics* Sequence Homology, Amino Acid Telomerase / antagonists & inhibitors* Tumor Suppressor Proteins / genetics* Zebrafish / genetics* Zebrafish Proteins / genetics*
IF 2.984
引用数 11
WOS 分野 GENETICS & HEREDITY
リソース情報
ヒト・動物細胞 BRF41(RCB0804)