RRC ID |
41315
|
著者 |
Sakai H, Sumitani M, Chikami Y, Yahata K, Uchino K, Kiuchi T, Katsuma S, Aoki F, Sezutsu H, Suzuki MG.
|
タイトル |
Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori.
|
ジャーナル |
PLoS Genet
|
Abstract |
In Bombyx mori (B. mori), Fem piRNA originates from the W chromosome and is responsible for femaleness. The Fem piRNA-PIWI complex targets and cleaves mRNAs transcribed from the Masc gene. Masc encodes a novel CCCH type zinc-finger protein and is required for male-specific splicing of B. mori doublesex (Bmdsx) transcripts. In the present study, several silkworm strains carrying a transgene, which encodes a Fem piRNA-resistant Masc mRNA (Masc-R), were generated. Forced expression of the Masc-R transgene caused female-specific lethality during the larval stages. One of the Masc-R strains weakly expressed Masc-R in various tissues. Females heterozygous for the transgene expressed male-specific isoform of the Bombyx homolog of insulin-like growth factor II mRNA-binding protein (ImpM) and Bmdsx. All examined females showed a lower inducibility of vitellogenin synthesis and exhibited abnormalities in the ovaries. Testis-like tissues were observed in abnormal ovaries and, notably, the tissues contained considerable numbers of sperm bundles. Homozygous expression of the transgene resulted in formation of the male-specific abdominal segment in adult females and caused partial male differentiation in female genitalia. These results strongly suggest that Masc is an important regulatory gene of maleness in B. mori.
|
巻・号 |
12(8)
|
ページ |
e1006203
|
公開日 |
2016-8-1
|
DOI |
10.1371/journal.pgen.1006203
|
PII |
PGENETICS-D-16-00853
|
PMID |
27579676
|
PMC |
PMC5007099
|
MeSH |
Animals
Animals, Genetically Modified
Bombyx / genetics*
Bombyx / growth & development
DNA-Binding Proteins / biosynthesis
DNA-Binding Proteins / genetics*
Female
Gene Expression Regulation, Developmental
Insect Proteins / biosynthesis
Insect Proteins / genetics*
Insulin-Like Growth Factor II / biosynthesis
Insulin-Like Growth Factor II / genetics*
Male
Ovary / growth & development
Ovary / metabolism
RNA Splicing / genetics
RNA, Small Interfering / biosynthesis
RNA, Small Interfering / genetics*
Sex Chromosomes / genetics
Sex Determination Processes / genetics*
Testis / growth & development
Testis / metabolism
Vitellogenins / biosynthesis
Vitellogenins / genetics
|
IF |
5.175
|
リソース情報 |
カイコ |
Suzu19-1 |