RRC ID 50266
著者 Ueki N, Wakabayashi KI.
タイトル Detergent-extracted Volvox model exhibits an anterior-posterior gradient in flagellar Ca2+ sensitivity.
ジャーナル Proc Natl Acad Sci U S A
Abstract Volvox rousseletii is a multicellular spheroidal green alga containing ∼5,000 cells, each equipped with two flagella (cilia). This organism shows striking photobehavior without any known intercellular communication. To help understand how the behavior of flagella is regulated, we developed a method to extract the whole organism with detergent and reactivate its flagellar motility. Upon addition of ATP, demembranated flagella (axonemes) in the spheroids actively beat and the spheroids swam as if they were alive. Under Ca2+-free conditions, the axonemes assumed planar and asymmetrical waveforms and beat toward the posterior pole, as do live spheroids in the absence of light stimulation. In the presence of 10-6 M Ca2+, however, most axonemes beat three-dimensionally toward the anterior pole, similar to flagella in photostimulated live spheroids. This Ca2+-dependent change in flagellar beating direction was more conspicuous near the anterior pole of the spheroid, but was not observed near the posterior pole. This anterior-posterior gradient of flagellar Ca2+ sensitivity may explain the mechanism of V. rousseletii photobehavior.
巻・号 115(5)
ページ E1061-E1068
公開日 2018-1-30
DOI 10.1073/pnas.1715489115
PII 1715489115
PMID 29311312
PMC PMC5798354
MeSH Adenosine Triphosphate / chemistry Axoneme / physiology Calcium / physiology* Detergents / chemistry* Flagella / physiology* Microscopy, Phase-Contrast Movement Photosynthesis Phototaxis* Video Recording Volvox / physiology*
IF 9.412
引用数 3
リソース情報
藻類 NIES-4029