| RRC ID |
12048
|
| 著者 |
Naiki T, Nagaki M, Shidoji Y, Kojima H, Moriwaki H.
|
| タイトル |
Functional activity of human hepatoma cells transfected with adenovirus-mediated hepatocyte nuclear factor (HNF)-4 gene.
|
| ジャーナル |
Cell Transplant
|
| Abstract |
Fulminant hepatic failure (FHF) is still associated with high mortality despite recent advances in medical management. There is need of an effective and safe bioartificial liver (BAL) support to help keep patients with FHF alive until an organ becomes available for transplantation or the native liver recovers. The aim of this study was to establish highly functional liver cells by means of transfecting hepatocyte nuclear factor (HNF)-4 gene for the development of BAL. We constructed adenovirus vector carrying rat HNF-4 cDNA, and transfected to hepatoma-derived cell lines, HepG2 and HuH-7, to enforce expression of the exogenous HNF-4 gene. We analyzed expression of HNF-4, HNF-1, and liver-specific genes in cells infected by the adenovirus vector expressing HNF-4. Adenovirus-mediated HNF-4 gene transfer resulted in increases in expressions of HNF-4, HNF-1, and liver-specific genes such as apolipoproteins, alpha1-antitrypsin (alpha1-AT), phosphoenolpyruvate carboxy-kinase, cytochrome P450 families, and glutamine synthetase in transfected hepatoma cells. Cells overexpressing HNF-4 removed ammonia from medium supplemented with NH4Cl to a greater extent than control cells. These findings demonstrated that transfected cell lines restored differentiated gene expressions and liver-specific function by the overproduction of HNF-4. HNF-4-overexpressing hepatocyte cell lines are useful for bioreactor of BAL systems.
|
| 巻・号 |
13(4)
|
| ページ |
393-403
|
| 公開日 |
2004-1-1
|
| DOI |
10.3727/000000004783983855
|
| PMID |
15468681
|
| MeSH |
Adenoviridae / genetics
Animals
Carcinoma, Hepatocellular
Cell Line, Tumor / enzymology
Cell Line, Tumor / metabolism*
Hepatoblastoma
Hepatocyte Nuclear Factor 4 / biosynthesis
Hepatocyte Nuclear Factor 4 / genetics*
Humans
Liver / cytology
Liver / enzymology
Liver / metabolism
Liver, Artificial
Rats
Transfection
|
| IF |
3.341
|
| 引用数 |
20
|
|
WOS 分野
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
CELL & TISSUE ENGINEERING
|
| リソース情報 |
| 遺伝子材料 |
AxCALacZ Adex1CAlacZ (RDB02726) |
| ヒト・動物細胞 |
Hep G2 |
