RRC ID |
12199
|
著者 |
Nakamura T, Mura T, Saito K, Ohsawa T, Akiyoshi H, Sato K.
|
タイトル |
Adenovirus-transferred HNF-3 gamma conserves some liver functions in primary cultured hepatocytes of adult rats.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Hepatocyte nuclear factor-3 (HNF-3) isoforms are key factors for regulation of gene expression and differentiation in hepatocytes. HNF-3gamma is abundantly expressed in the mature liver, but down-regulated in primary cultured hepatocytes, in which some other hepatic gene expressions are also decreased. In this study, the primary hepatocytes were infected with the recombinant adenovirus carrying HNF-3gamma gene (AxCAHNF3gamma), and this led to marked induction of the HNF-3gamma gene. As a result, the expressions of albumin, catalase, and ornithine transcarbamylase (OTC) genes were also recovered to significant levels in the AxCAHNF3gamma-infected hepatocytes. Moreover, hepatocyte proliferation stimulated by epidermal growth factor (EGF) and insulin was also inhibited by AxCAHNF3gamma infection. Our results demonstrate that the enforced expression of HNF-3gamma gene can lead to conservation of some original liver functions in the primary cultured hepatocytes accompanied by morphological differentiation and growth inhibition.
|
巻・号 |
253(2)
|
ページ |
352-7
|
公開日 |
1998-12-18
|
DOI |
10.1006/bbrc.1998.9797
|
PII |
S0006-291X(98)99797-2
|
PMID |
9878541
|
MeSH |
Adenoviridae / genetics*
Animals
Cell Differentiation / genetics
Cells, Cultured
DNA-Binding Proteins / biosynthesis
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / physiology
Down-Regulation / genetics
Gene Expression Regulation
Gene Transfer Techniques*
Hepatocyte Nuclear Factor 3-gamma
Liver / cytology*
Liver / metabolism
Liver / physiology*
Male
Nuclear Proteins / biosynthesis
Nuclear Proteins / genetics*
Nuclear Proteins / physiology
Rats
Rats, Wistar
Recombination, Genetic
Transcription Factors / biosynthesis
Transcription Factors / genetics*
Transcription Factors / physiology
|
IF |
2.985
|
引用数 |
13
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
遺伝子材料 |
AxCALacZ Adex1CAlacZ (RDB02726) |