RRC ID 12762
著者 Fujita K, Yamaguchi Y, Mori T, Muramatsu N, Miyamoto T, Yano M, Miyata H, Ootsuyama A, Sawada M, Matsuda H, Kaji R, Sakaguchi S.
タイトル Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions.
ジャーナル Cell Mol Neurobiol
Abstract We first verified that a single chain Fv fragment against prion protein (anti-PrP scFv) was secreted by HEK293T cells and prevented prion replication in infected cells. We then stably expressed anti-PrP scFv in brain-engraftable murine microglial cells and intracerebrally injected these cells into mice before or after infection with prions. Interestingly, the injection before or at an early time point after infection attenuated the infection marginally but significantly prolonged survival times of the mice. These suggest that the ex vivo gene transfer of anti-PrP scFvs using brain-engraftable cells could be a possible immunotherapeutic approach against prion diseases.
巻・号 31(7)
ページ 999-1008
公開日 2011-10-1
DOI 10.1007/s10571-011-9696-z
PMID 21516351
MeSH Animals Brain / cytology* Cell Line Genetic Vectors HEK293 Cells Humans Mice Microglia / cytology Microglia / physiology* Microglia / transplantation* Prions / immunology* Prions / pathogenicity Prions / physiology Recombinant Proteins / genetics Recombinant Proteins / immunology Scrapie / physiopathology* Scrapie / therapy Single-Chain Antibodies / genetics Single-Chain Antibodies / immunology* Single-Chain Antibodies / therapeutic use Survival Rate
IF 3.606
引用数 12
WOS 分野 NEUROSCIENCES CELL BIOLOGY
リソース情報
遺伝子材料 CS-CA-GFP (RDB05964) CS-CA-MCS (RDB05963) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)