| RRC ID |
1431
|
| 著者 |
Kanao H, Enomoto T, Kimura T, Fujita M, Nakashima R, Ueda Y, Ueno Y, Miyatake T, Yoshizaki T, Buzard GS, Tanigami A, Yoshino K, Murata Y.
|
| タイトル |
Overexpression of LAMP3/TSC403/DC-LAMP promotes metastasis in uterine cervical cancer.
|
| ジャーナル |
Cancer Res
|
| Abstract |
LAMP3 (DC-LAMP, TSC403, CD208) was originally isolated as a gene specifically expressed in lung tissues. LAMP3 is located on a chromosome 3q segment that is frequently amplified in some human cancers, including uterine cervical cancer. Because two other members of the LAMP family of lysosomal membrane glycoproteins, LAMP1 and LAMP2, were previously implicated in potentially modulating the interaction of vascular endothelial and cancer cells, we hypothesized that LAMP3 might also play an important part in metastasis. To clarify the metastatic potential of LAMP3 in cervical cancers, we transfected a LAMP3 expression vector into a human uterine cervical cancer cell line, TCS. In an in vitro invasion assay, the migration of LAMP3-overexpressing TCS cells was significantly higher than in control TCS cells. In an in vivo metastasis assay, distant metastasis was detected in 9 of 11 LAMP3-overexpressing TCS cell-injected mice and in only 1 of 11 control mice. Histologic study showed that LAMP3-overexpressing cells readily invaded into the lymph-vascular space. In clinical samples, quantitative real-time reverse transcription-PCR (RT-PCR) analyses showed that LAMP3 mRNA was significantly up-regulated in 47 of 47 (100%) cervical cancers and in 2 of 15 (13%) cervical intraepithelial neoplasias, compared with a low level of LAMP3 mRNA expressed in normal uterine cervixes. Interestingly, high LAMP3 expression was significantly correlated with the overall survival of patients with stage I/II cervical cancers. These findings indicate that LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer.
|
| 巻・号 |
65(19)
|
| ページ |
8640-5
|
| 公開日 |
2005-10-1
|
| DOI |
10.1158/0008-5472.CAN-04-4112
|
| PII |
65/19/8640
|
| PMID |
16204031
|
| MeSH |
Animals
Cell Movement / genetics
Female
Gene Expression
Humans
Immunohistochemistry
Lysosome-Associated Membrane Glycoproteins / biosynthesis*
Lysosome-Associated Membrane Glycoproteins / genetics*
Mice
Mice, SCID
Neoplasm Metastasis
Neoplasm Proteins / biosynthesis*
Neoplasm Proteins / genetics*
Neoplasm Staging
Neoplasm Transplantation
Oncogenes / genetics
Prognosis
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
Transfection
Transplantation, Heterologous
Uterine Cervical Neoplasms / genetics*
Uterine Cervical Neoplasms / metabolism*
Uterine Cervical Neoplasms / pathology
|
| IF |
9.727
|
| 引用数 |
61
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
TCS(RCB0638) |
