RRC ID 1436
著者 Masuda S, Murakami M, Takanezawa Y, Aoki J, Arai H, Ishikawa Y, Ishii T, Arioka M, Kudo I.
タイトル Neuronal expression and neuritogenic action of group X secreted phospholipase A2.
ジャーナル J Biol Chem
Abstract Although individual mammalian secreted phospholipase A(2) (sPLA(2)) enzymes exhibit unique tissue and cellular distributions, the cell type-specific functions of each enzyme remain largely unknown. In this study, we found by immunohistochemistry that group X sPLA(2) (sPLA(2)-X) is uniquely located in the peripheral neuronal fibers, an observation that was supported by detection of its transcript and protein in the neuronal cell line PC12 and in primary dorsal root ganglia neurons. Adenoviral expression of sPLA(2)-X in PC12 cells facilitated neurite outgrowth, particularly when combined with a suboptimal concentration of nerve growth factor. In neuronally differentiated PC12 cells, sPLA(2)-X was preferentially localized in the Golgi apparatus and growth cones, and proteolytic conversion of the proenzyme to mature enzyme mainly occurred after the secretion process. The neurite-extending ability of sPLA(2)-X depended on the production of its catalytic product, lysophosphatidylcholine. Moreover, nerve growth factor-induced neurite extension of PC12 cells was modestly but significantly attenuated by an anti-sPLA(2)-X antibody or by a small interfering RNA for sPLA(2)-X. These observations suggest the potential contribution of sPLA(2)-X to neuronal differentiation, and possibly repair, under certain conditions.
巻・号 280(24)
ページ 23203-14
公開日 2005-6-17
DOI 10.1074/jbc.M500985200
PII S0021-9258(20)61495-8
PMID 15781456
MeSH Adenoviridae / metabolism Animals Blotting, Northern Catalysis Cell Differentiation Cell Line Chromatography, Thin Layer DNA, Complementary / metabolism Ganglia, Spinal / metabolism Golgi Apparatus / metabolism Humans Immunoblotting Immunohistochemistry Lysophosphatidylcholines / metabolism Mice Mice, Inbred C57BL Microscopy, Confocal Nerve Growth Factor Neurons / enzymology* Neurons / metabolism PC12 Cells Phospholipases A / biosynthesis* Phospholipases A / metabolism* Phospholipases A2 RNA, Small Interfering / metabolism Rats Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Mass, Electrospray Ionization Time Factors
IF 4.238
引用数 49
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 PC-12(RCB0009)