RRC ID 28595
著者 Carr SB, Mecia LB, Phillips SE, Thomas CD.
タイトル Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1.
ジャーナル Acta Crystallogr Sect F Struct Biol Cryst Commun
Abstract Antibiotic resistance in bacterial pathogens poses an ever-increasing risk to human health. In antibiotic-resistant strains of Staphylococcus aureus this resistance often resides in extra-chromosomal plasmids, such as those of the pT181 family, which replicate via a rolling-circle mechanism mediated by a plasmid-encoded replication initiation protein. Currently, there is no structural information available for the pT181-family Rep proteins. Here, the crystallization of a catalytically active fragment of a homologous replication initiation protein from the thermophile Geobacillus stearothermophilus responsible for the replication of plasmid pSTK1 is reported. Crystals of the RepSTK1 fragment diffracted to a resolution of 2.5 Å and belonged to space group P2₁2₁2₁.
巻・号 69(Pt 10)
ページ 1123-6
公開日 2013-10-1
DOI 10.1107/S1744309113023828
PII S1744309113023828
PMID 24100563
PMC PMC3792671
MeSH DNA Helicases / chemistry* Electrophoresis, Polyacrylamide Gel Geobacillus stearothermophilus / metabolism* Humans Plasmids / metabolism* Trans-Activators / chemistry* X-Ray Diffraction*
IF 0.968
引用数 1
WOS 分野 CRYSTALLOGRAPHY BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY BIOCHEMICAL RESEARCH METHODS
リソース情報
遺伝子材料 pSTE33 (RDB00920)