RRC ID 29127
著者 Sato K, Nakano A.
タイトル Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae.
ジャーナル Mol Biol Cell
Abstract The yeast open reading frame YLR080w/EMP46 encodes a homolog of the Golgi protein Emp47p. These two proteins are 45% identical and have a single transmembrane domain in their C-terminal regions and a carbohydrate recognition domain signature in the N-terminal region. The C-terminal tail of Emp46p includes a dilysine signal. This protein is localized to Golgi membranes at steady state by subcellular fractionation and green fluorescent protein labeling. On block of forward transport in sec12-4 cells, redistribution of Emp46p from the Golgi to the endoplasmic reticulum is observed. These localization features are similar to those previously reported for Emp47p. In addition, mutagenesis of the C-terminal region identified a tyrosine-containing motif as a critical determinant of the Golgi-localization and interaction with both COPI and COPII components. Similar motifs are also observed in the C-terminal tail of Emp47p and other mammalian homologs. Disruption of Emp47p displays a growth defect at a high temperature or on Ca(2+)-containing medium, which is rescued by overexpression of Emp46p, suggesting a partially overlapping function between Emp46p and Emp47p. In addition, we found that the disruption of both Emp46p and Emp47p show a marked defect in the secretion of a subset of glycoproteins. Analysis of the C-terminal mutants for Ca(2+) sensitivity revealed that the forward transport of Emp46/47p is essential for their function, whereas the retrograde transport is not. We propose that Emp46p and Emp47p are required for the export of specific glycoprotein cargo from the endoplasmic reticulum.
巻・号 13(7)
ページ 2518-32
公開日 2002-7-1
DOI 10.1091/mbc.e02-01-0027
PMID 12134087
PMC PMC117331
MeSH Amino Acid Sequence Animals COP-Coated Vesicles / metabolism Cell Fractionation Endoplasmic Reticulum / metabolism* Gene Deletion Genes, Reporter Glycoproteins / genetics Glycoproteins / metabolism* Golgi Apparatus / metabolism Humans Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Open Reading Frames Phenotype Protein Sorting Signals* Protein Structure, Tertiary Protein Transport / physiology Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Tyrosine / metabolism* Vesicular Transport Proteins
IF 3.791
引用数 77
WOS 分野 CELL BIOLOGY
リソース情報
遺伝子材料 pKSY103 (RDB08665) pKSY104 (RDB08666) pKSY105 (RDB08667) pKSY113 (RDB08668) pKSY114 (RDB08669) pKSY115 (RDB08670) pKSY116 (RDB08671) pKSY117 (RDB08672) pKSY118 (RDB08673) pKSY121 (RDB08674) pKSY122 (RDB08675) pKSY123 (RDB08676) pKSY126 (RDB08677) pKSY131 (RDB08678) pKSY132 (RDB08679) pKSY133 (RDB08680) pKSY134 (RDB08681) pKSY135 (RDB08682) pKSE106 (RDB08693) pKSE107 (RDB08694) pKSE108 (RDB08695) pKSE112 (RDB08696) pKSE113 (RDB08697) pKSE114 (RDB08698) pKSE115 (RDB08699) pKSE116 (RDB08700) pKSE117 (RDB08701) pKSE121 (RDB08702) pKSE122 (RDB08703)