RRC ID 29132
著者 Kimura N, Fukushima A, Oda K, Nakada S.
タイトル An in vivo study of the replication origin in the influenza virus complementary RNA.
ジャーナル J Biochem
Abstract A new in vivo replication system for influenza virus was developed by using the clone 76 cell line, in which the viral RNA polymerase and nucleoprotein genes can be expressed in response to dexamethasone. The chimeric NS-chloramphenicol acetyltransferase (CAT) RNAs in the sense and antisense orientations positioned between the 5'- and 3'-terminal sequences of the influenza virus RNA segment 8 can be replicated [both genomic RNA (vRNA) and complementary RNA (cRNA) were transcribed] in the clone 76 cells treated with dexamethasone. These data indicate that three RNA polymerase proteins (PB1, PB2, and PA) and nucleoprotein are sufficient for replication of the influenza virus genome. Analysis of mutant cRNAs containing a base-substitution or a deletion in the 3'-conserved terminal 13 nucleotides revealed that important cis elements in the cRNA for vRNA synthesis reside at positions 2, 3, and 7 to 13 nucleotides from the 3'-end.
巻・号 113(1)
ページ 88-92
公開日 1993-1-1
DOI 10.1093/oxfordjournals.jbchem.a124009
PMID 7681056
MeSH Animals Base Sequence Blotting, Northern Cell Line Chloramphenicol O-Acetyltransferase / genetics Cloning, Molecular Dexamethasone / pharmacology Influenza A virus / genetics* Influenza A virus / physiology Mice Molecular Sequence Data Mutation Nucleic Acid Conformation Polymerase Chain Reaction RNA / biosynthesis RNA / genetics RNA, Antisense RNA, Complementary RNA, Viral / biosynthesis RNA, Viral / genetics* Recombinant Fusion Proteins / genetics Transcription, Genetic Viral Nonstructural Proteins / genetics Virus Replication / genetics*
IF 2.476
引用数 10
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 pT7/NS-CATc (RDB02055) pT7/NS-CATc-mut (1U-C) (RDB02056) pT7/NS-CATc-mut (2C-G) (RDB02057) pT7/NS-CATc-mut (3A-C) (RDB02058) pT7/NS-CATc-mut (5C-A) (RDB02059) pT7/NS-CATc-mut (6U-A) (RDB02060) pT7/NS-CATc-mut (7U-A) (RDB02061) pT7/NS-CATc-mut (8U-C) (RDB02062) pT7/NS-CATc-mut (9G-C) (RDB02063) pT7/NS-CATc-mut (10U-delta) (RDB02064) pT7/NS-CATc-mut (11U-A) (RDB02065) pT7/NS-CATc-mut (12C-G) (RDB02066) pT7/NS-CATc-mut (13C-G) (RDB02067) pT7/delta NSc (RDB02068)