RRC ID 30207
著者 Minoura I, Hachikubo Y, Yamakita Y, Takazaki H, Ayukawa R, Uchimura S, Muto E.
タイトル Overexpression, purification, and functional analysis of recombinant human tubulin dimer.
ジャーナル FEBS Lett
Abstract Microtubules consisting of tubulin dimers play essential roles in various cellular functions. Investigating the structure-function relationship of tubulin dimers requires a method to prepare sufficient quantities of recombinant tubulin. To this end, we simultaneously expressed human α1- and β3-tubulin using a baculovirus-insect cell expression system that enabled the purification of 5mg recombinant tubulin per litre of cell culture. The purified recombinant human tubulin could be polymerized into microtubules that glide on a kinesin-coated glass surface. The method provides a powerful tool for in vitro functional analyses of microtubules.
巻・号 587(21)
ページ 3450-5
公開日 2013-11-1
DOI 10.1016/j.febslet.2013.08.032
PII S0014-5793(13)00661-3
PMID 24021646
MeSH Cells, Cultured Humans Kinesins / metabolism Protein Multimerization Recombinant Proteins / genetics* Recombinant Proteins / isolation & purification* Recombinant Proteins / metabolism Tubulin / genetics* Tubulin / isolation & purification* Tubulin / metabolism
IF 3.057
引用数 50
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
遺伝子材料 pFBD-Hs alpha1CGH/beta3CGF (RDB12753) pFBD-Hs alpha1(K40R)CGH/beta3CGF (RDB12754).