RRC ID |
30899
|
著者 |
Shibahara S, Tomita Y, Tagami H, Müller RM, Cohen T.
|
タイトル |
Molecular basis for the heterogeneity of human tyrosinase.
|
ジャーナル |
Tohoku J Exp Med
|
Abstract |
A cDNA clone, pHT gamma 1, representing human tyrosinase mRNA was isolated by screening a melanoma cDNA library with a synthetic oligonucleotide complementary to a segment of the human tyrosinase cDNA, Pmel 34 [Kwon et al. (1987) Proc. nat. Acad. Sci. USA 84, 7473-7477]. However, there are a number of differences in the nucleotide sequence between two cDNAs, pHT gamma 1 and Pmel 34, particularly in the region coding for the carboxyl terminus of the enzyme (putative exon 5). We therefore cloned the genomic DNA segment carrying the exon 5 of the human tyrosinase gene by screening a human placental genomic DNA library with a cloned cDNA probe. The nucleotide sequences of human tyrosinase cDNA as well as part of its gene were determined. Mature human tyrosinase is composed of 511 amino acids with a molecular weight of 58,000. We provide evidence for the presence of at least two species of human tyrosinase mRNA generated by alternative splicing in human pigmented melanoma cells.
|
巻・号 |
156(4)
|
ページ |
403-14
|
公開日 |
1988-12-1
|
DOI |
10.1620/tjem.156.403
|
PMID |
2854305
|
MeSH |
Amino Acid Sequence
Base Sequence
Catechol Oxidase / genetics*
Cloning, Molecular
DNA / isolation & purification
DNA, Recombinant / analysis
Endonucleases
Humans
Melanoma / analysis
Melanoma / pathology
Molecular Sequence Data
Monophenol Monooxygenase / analysis
Monophenol Monooxygenase / genetics*
RNA, Messenger / analysis
Restriction Mapping
Sequence Homology, Nucleic Acid
Single-Strand Specific DNA and RNA Endonucleases
Tumor Cells, Cultured / analysis
|
IF |
1.441
|
引用数 |
90
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
リソース情報 |
遺伝子材料 |
pHT gamma 1 (RDB01205)
pRHOHT2 (RDB02082) |