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Reference detail
Reference Info.
Pubmed ID 25258322  
RRC ID 33252 
Author Machida K, Mikami S, Masutani M, Mishima K, Kobayashi T, Imataka H. 
Title A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. 
Journal J Biol Chem. 
Published 2014-11-14 
Volume 289(46) 
Pages 31960-71 
DOI 10.1074/jbc.M114.593343   
PII M114.593343 
Alanine / genetics
Amino Acyl-tRNA Synthetases / metabolism
Binding Sites
Cell-Free System
DNA, Complementary / metabolism
Encephalomyocarditis virus / metabolism*
Eukaryotic Initiation Factor-2 / metabolism*
Gene Deletion
Gene Expression Regulation
HeLa Cells
Hepacivirus / metabolism
Open Reading Frames
Peptide Elongation Factor 1 / metabolism*
Plasmids / metabolism
Protein Biosynthesis*
Protein Folding
Ribosomes / chemistry
Viral Nonstructural Proteins / chemistry*
Viral Nonstructural Proteins / metabolism*
Viral Proteins / metabolism*
Resource Info.
Species DNA material 
Resource name
pUC-T7-HCV HA-2A-2B-FLAG (RDB13261); pUC-T7-HCV HA-2A-2B-HA (RDB13262); pUC-T7-HCV HA-2A(silent-1)2B-HA (RDB13263); pUC-T7-HCV HA-2A(silent-2)2B-HA (RDB13264); pUC-T7-HCV HA-2A(uORF2) (RDB13265); pUC-T7-EMCV-His-eEF1A (RDB13266); pUC-T7-EMCV-His-eEF1Bgamma (RDB13267); pUC-T7-EMCV-eEF1Balpha (RDB13268); pGEX6P-eRF1 (RDB13269); pGEX6P-eRF3 (RDB13270); pUC-T7-EMCV-GST-Glu-ProRS (RDB13271); pUC-T7-EMCV-GST-MetRS (RDB13272); pUC-T7-EMCV-GST-AspRS (RDB13273); pUC-T7-EMCV-GST-AlaRS (RDB13274); pUC-T7-EMCV-GST-CysRS (RDB13275); pUC-T7-EMCV-GST-ValRS (RDB13276); pUC-T7-EMCV-GST-TyrRS (RDB13277); pUC-T7-EMCV-His-PheRSbeta (RDB13278); pUC-T7-HCV-Rluc-HA (RDB13279); pUC-T7-HCV-beta-actin-HA (RDB13280); pUC-T7-HCV-PABP-HA (RDB13281); pUC-T7-HCV-beta-Gal-HA (RDB13282); pUC-T7-HCV-HA-Rluc (RDB13283); pUC-T7-HCV-HA-beta-actin (RDB13284); pUC-T7-HCV-HA-PABP (RDB13285); pUC-T7-HCV-HA-beta-Gal (RDB13286); pUC-T7-HCV HA-2A-HA-2B (22 aa) (RDB13287). 

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