| RRC ID |
35683
|
| 著者 |
Maruyama T, Kusakari S, Sato-Hashimoto M, Hayashi Y, Kotani T, Murata Y, Okazawa H, Oldenborg PA, Kishi S, Matozaki T, Ohnishi H.
|
| タイトル |
Hypothermia-induced tyrosine phosphorylation of SIRPα in the brain.
|
| ジャーナル |
J Neurochem
|
| Abstract |
Signal regulatory protein α (SIRPα) is a neuronal membrane protein that undergoes tyrosine phosphorylation in the brain of mice in response to forced swim (FS) stress in cold water, and this response is implicated in regulation of depression-like behavior in the FS test. We now show that subjection of mice to the FS in warm (37 °C) water does not induce the tyrosine phosphorylation of SIRPα in the brain. The rectal temperature (T(rec) ) of mice was reduced to 27° to 30 °C by performance of the FS for 10 min in cold water, whereas it was not affected by the same treatment in warm water. The level of tyrosine phosphorylation of SIRPα in the brain was increased by administration of ethanol or picrotoxin, starvation, or cooling after anesthesia, all of which also induced hypothermia. Furthermore, the tyrosine phosphorylation of SIRPα in cultured hippocampal neurons was induced by lowering the temperature of the culture medium. CD47, a ligand of SIRPα, as well as Src family kinases or SH2 domain-containing protein phosphatase 2 (Shp2), might be important for the basal and the hypothermia-induced tyrosine phosphorylation of SIRPα. Hypothermia is therefore likely an important determinant of both the behavioral immobility and tyrosine phosphorylation of SIRPα observed in the FS test.
|
| 巻・号 |
121(6)
|
| ページ |
891-902
|
| 公開日 |
2012-6-1
|
| DOI |
10.1111/j.1471-4159.2012.07748.x
|
| PMID |
22468987
|
| MeSH |
Animals
Brain / metabolism*
Hypothermia / metabolism*
Immunoblotting
Immunohistochemistry
Immunoprecipitation
Mice
Mice, Inbred C57BL
Mice, Knockout
Neurons / metabolism*
Phosphorylation
RNA Interference
Receptors, Immunologic / metabolism*
Stress, Psychological / metabolism
Tyrosine / metabolism
|
| IF |
4.066
|
| 引用数 |
4
|
|
WOS 分野
|
NEUROSCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393)
CS-CDF-EG-PRE-RfA (RDB07722). |
