RRC ID |
35791
|
著者 |
Ohnishi T, Shirane M, Hashimoto Y, Saita S, Nakayama KI.
|
タイトル |
Identification and characterization of a neuron-specific isoform of protrudin.
|
ジャーナル |
Genes Cells
|
Abstract |
Protrudin is a membrane protein that regulates polarized vesicular transport. Now, we have identified a novel isoform of protrudin (protrudin-L) that contains an additional seven amino acids between the FFAT motif and the coiled-coil domain compared with the conventional isoform (protrudin-S) as a result of alternative splicing of a microexon (exon L). Protrudin-L mRNA was found to be mostly restricted to the central nervous system in mice, whereas protrudin-S mRNA was detected in all tissues examined. With the use of a splicing reporter minigene that produces two distinct fluorescent proteins in a manner dependent on the splicing pattern of protrudin transcripts, we found that most neurons express protrudin-L, whereas astrocytes express both protrudin isoforms and oligodendrocytes express only protrudin-S. Protrudin-L associated to a greater extent with vesicle-associated membrane protein-associated protein (VAP) than protrudin-S. Expression of protrudin-L in hippocampal neurons of protrudin-deficient mice also promoted neurite outgrowth more efficiently than protrudin-S. Our results suggest that protrudin-L is a neuron-specific protrudin isoform that promotes axonal elongation and contributes to the establishment of neuronal polarity.
|
巻・号 |
19(2)
|
ページ |
97-111
|
公開日 |
2014-2-1
|
DOI |
10.1111/gtc.12109
|
PMID |
24251978
|
MeSH |
Alternative Splicing
Animals
Cell Line, Tumor
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Neurons / metabolism*
Organ Specificity
Protein Isoforms / genetics
Protein Isoforms / metabolism
R-SNARE Proteins / genetics
R-SNARE Proteins / metabolism*
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*
|
IF |
1.655
|
引用数 |
13
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
|
リソース情報 |
遺伝子材料 |
CSII-CMV-MCS (RDB04377)
pCMV-VSV-G-RSV-Rev (RDB04393)
pCAG-HIVgp (RDB04394). |