RRC ID 35799
著者 Donai K, Kiyono T, Eitsuka T, Guo Y, Kuroda K, Sone H, Isogai E, Fukuda T.
タイトル Bovine and porcine fibroblasts can be immortalized with intact karyotype by the expression of mutant cyclin dependent kinase 4, cyclin D, and telomerase.
ジャーナル J Biotechnol
Abstract Cattle and pigs comprise the most economically important livestock. Despite their importance, cultured cells from these species, which are useful for physiological analyses, are quite limited in cell banks. One of the reasons for the limited number of cell lines is the difficulty in their establishment. To overcome limitations in cell-line establishment, we attempted to immortalize bovine and porcine fibroblasts by transduction of multiple cell cycle regulators (mutant cyclin dependent kinase 4, cyclin D and telomerase reverse transcriptase). The transduced cells continued to display a stable proliferation rate and did not show cellular senescence. Furthermore, cell cycle assays showed that induction of these exogenous genes enhanced turnover of the cell cycle, especially at the G1-S phase. Furthermore, our established cell lines maintained normal diploid karyotypes at 98-100%. Our study demonstrated that bypassing p16/Rb-mediated cell arrest and activation of telomerase activity enabled efficient establishment of immortalized bovine- and porcine-derived fibroblasts. The high efficiency of establishing cell lines suggests that the networks of cell cycle regulators, especially p16/Rb-associated cell cycle arrest, have been conserved during evolution of humans, cattle, and pigs.
巻・号 176
ページ 50-7
公開日 2014-4-20
DOI 10.1016/j.jbiotec.2014.02.017
PII S0168-1656(14)00090-X
PMID 24589663
MeSH Animals Cattle Cell Cycle Cell Line* Cell Proliferation Cyclin D / genetics Cyclin D / metabolism* Cyclin-Dependent Kinase 4 / genetics Cyclin-Dependent Kinase 4 / metabolism* Diploidy Embryo, Mammalian / cytology* Fibroblasts / cytology* Fibroblasts / metabolism Karyotype Swine Telomerase / genetics Telomerase / metabolism* Transduction, Genetic
IF 3.503
引用数 24
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
遺伝子材料 CSII-CMV-RfA (RDB04386) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394).