RRC ID 38212
著者 Yoshimura A, Fujii R, Watanabe Y, Okabe S, Fukui K, Takumi T.
タイトル Myosin-Va facilitates the accumulation of mRNA/protein complex in dendritic spines.
ジャーナル Curr Biol
Abstract mRNA localization has an essential role in localizing cytoplasmic determinants, controlling the direction of protein secretion, and allowing the local control of protein synthesis in neurons. In neuronal dendrites, the localization and translocation of mRNA is considered as one of the molecular bases of synaptic plasticity. Recent imaging and functional studies revealed that several RNA-binding proteins form a large messenger ribonucleoprotein (mRNP) complex that is involved in transport and translation of mRNA in dendrites. However, the mechanism of mRNA translocation into dendritic spines is unknown. Here, we show that an actin-based motor, myosin-Va, plays a significant role in mRNP transport in neuronal dendrites and spines. Myosin-Va was Ca2+-dependently associated with TLS, an RNA-binding protein, and its target RNA Nd1-L, an actin stabilizer. A dominant-negative mutant or RNAi of myosin-Va in neurons suppressed TLS accumulation in spines and further impaired TLS dynamics upon activation of mGluRs. The TLS translocation into spines was impeded also in neurons prepared from myosin-Va-null dilute-lethal (dl) mice, which exhibit neurological defects. Our results demonstrate that myosin-Va facilitates the transport of TLS-containing mRNP complexes in spines and may function in synaptic plasticity through Ca2+ signaling.
巻・号 16(23)
ページ 2345-51
公開日 2006-12-5
DOI 10.1016/j.cub.2006.10.024
PII S0960-9822(06)02360-8
PMID 17141617
MeSH Animals Calcium / metabolism Calcium Signaling Dendritic Spines / metabolism* Intracellular Signaling Peptides and Proteins Mice Myosin Heavy Chains / physiology* Myosin Type V / physiology* Protein Transport Proteins / genetics RNA Interference RNA, Messenger / metabolism* RNA-Binding Protein FUS / metabolism Ribonucleoproteins / metabolism*
IF 9.601
引用数 127
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
遺伝子材料 pEGFP-MyosinVa_Full (RDB14185) pEGFP-MyosinVa_BRST (RDB14186) pEGFP-MyosinVa_BRSTK (RDB14187) pEGFP-MyosinVa_GTD (RDB14188).