RRC ID 47087
著者 Makino A, Abe M, Murate M, Inaba T, Yilmaz N, Hullin-Matsuda F, Kishimoto T, Schieber NL, Taguchi T, Arai H, Anderluh G, Parton RG, Kobayashi T.
タイトル Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.
ジャーナル FASEB J
Abstract Sphingomyelin (SM) is a major sphingolipid in mammalian cells and is reported to form specific lipid domains together with cholesterol. However, methods to examine the membrane distribution of SM are limited. We demonstrated in model membranes that fluorescent protein conjugates of 2 specific SM-binding toxins, lysenin (Lys) and equinatoxin II (EqtII), recognize different membrane distributions of SM; Lys exclusively binds clustered SM, whereas EqtII preferentially binds dispersed SM. Freeze-fracture immunoelectron microscopy showed that clustered but not dispersed SM formed lipid domains on the cell surface. Glycolipids and the membrane concentration of SM affect the SM distribution pattern on the plasma membrane. Using derivatives of Lys and EqtII as SM distribution-sensitive probes, we revealed the exclusive accumulation of SM clusters in the midbody at the time of cytokinesis. Interestingly, apical membranes of differentiated epithelial cells exhibited dispersed SM distribution, whereas SM was clustered in basolateral membranes. Clustered but not dispersed SM was absent from the cell surface of acid sphingomyelinase-deficient Niemann-Pick type A cells. These data suggest that both the SM content and membrane distribution are crucial for pathophysiological events bringing therapeutic perspective in the role of SM membrane distribution.
巻・号 29(2)
ページ 477-93
公開日 2015-2-1
DOI 10.1096/fj.13-247585
PII fj.13-247585
PMID 25389132
MeSH Animals COS Cells Cell Membrane / metabolism Cell Polarity Cell Survival Chlorocebus aethiops Cytokinesis / physiology* DNA, Complementary / metabolism Enzyme-Linked Immunosorbent Assay Epithelial Cells / cytology Fibroblasts / metabolism HeLa Cells Humans Infant Liposomes / metabolism Male Microscopy, Atomic Force Microscopy, Confocal Microscopy, Immunoelectron Niemann-Pick Disease, Type A / genetics Recombinant Proteins / metabolism Sphingomyelins / metabolism*
IF 4.966
引用数 41
WOS 分野 BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
遺伝子材料 pCAG/tdiRFP-CAAX (RDB13501). pET28/EqtII-EGFP-His6 (RDB13496) pET28/EqtII(8-69)-EGFP-His6 (RDB13497) pET28/His6-EGFP-NT-Lys (RDB13498) pET28/His6-mCherry-NT-Lys (RDB13499)