RRC ID 48098
著者 Shigemoto R, Yokota Y, Tsuchida K, Nakanishi S.
タイトル Cloning and expression of a rat neuromedin K receptor cDNA.
ジャーナル J Biol Chem
Abstract Functional cDNA clones for rat neuromedin K receptor were isolated from a rat brain cDNA library by cross-hybridization with the bovine substance K receptor cDNA. Injection of the mRNA synthesized in vitro from the cloned cDNA into Xenopus oocytes elicited electrophysiological responses to tachykinins, with the most potent sensitivity being to neuromedin K. Ligand-binding displacement in membranes of mammalian COS cells transfected with the cDNA indicated the rank order of affinity of the receptor to tachykinins: neuromedin K greater than substance K greater than substance P. The hybridization analysis showed that the neuromedin K receptor mRNA is expressed in both the brain and the peripheral tissues at different levels. The rat neuromedin K receptor consists of 452 amino acid residues and belongs to the family of G protein-coupled receptors, which are though to have seven transmembrane domains. The sequence comparison of the rat neuromedin K, substance P, and substance K receptors revealed that these receptors are highly conserved in the seven transmembrane domains and the cytoplasmic sides of the receptors. They also show some structural characteristics, including the common presence of histidine residues in transmembrane segments V and VI and the difference in the numbers and distributions of serine and threonine residues as possible phosphorylation sites in the cytoplasmic regions. This paper thus presents the first comprehensive analysis of the molecular nature of the multiple peptide receptors that exhibit similar but pharmacologically distinguishable activities.
巻・号 265(2)
ページ 623-8
公開日 1990-1-15
PII S0021-9258(19)40095-1
PMID 2153106
MeSH Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA / genetics* Gene Expression* Molecular Sequence Data Neurokinin B / metabolism Nucleic Acid Hybridization RNA, Messenger / genetics Rats Receptors, Neurokinin-3 Receptors, Neurotransmitter / genetics* Receptors, Neurotransmitter / metabolism Receptors, Tachykinin Transfection Xenopus / genetics
IF 4.238
引用数 302
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 pTKR 3 (RDB13181)