RRC ID |
48104
|
著者 |
Tanaka K, Masu M, Nakanishi S.
|
タイトル |
Structure and functional expression of the cloned rat neurotensin receptor.
|
ジャーナル |
Neuron
|
Abstract |
A functional cDNA clone for the rat neurotensin receptor was isolated by combining molecular cloning in an RNA expression vector with an electrophysiological assay in Xenopus oocytes. The neurotensin receptor consists of 424 amino acids with seven putative transmembrane domains and belongs to the family of G protein-coupled receptors. The cloned receptor expressed in mammalian cells or in Xenopus oocytes shows a selective and high-affinity binding to neurotensin peptides and undergoes potent desensitization by repeated application of neurotensin. The neurotensin receptor mRNA is expressed in both the brain and the peripheral tissues at different levels. This investigation discloses the molecular nature of the neurotensin receptor, which mediates the diverse neuronal and peripheral actions of neurotensin by effecting the G protein-associated second messenger system.
|
巻・号 |
4(6)
|
ページ |
847-54
|
公開日 |
1990-6-1
|
DOI |
10.1016/0896-6273(90)90137-5
|
PII |
0896-6273(90)90137-5
|
PMID |
1694443
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Binding, Competitive
Cell Line
Cloning, Molecular / methods
Female
GTP-Binding Proteins / genetics
GTP-Binding Proteins / metabolism
Gene Expression
Kinetics
Molecular Sequence Data
Neurotensin / metabolism*
Nucleic Acid Hybridization
Oocytes / metabolism
Organ Specificity
Poly A / genetics
RNA / genetics
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Receptors, Neurotensin
Receptors, Neurotransmitter / genetics*
Receptors, Neurotransmitter / metabolism
Recombinant Proteins / metabolism
Sequence Homology, Nucleic Acid
Xenopus laevis
|
IF |
14.415
|
引用数 |
485
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
遺伝子材料 |
prNTR 2 ( full8-6 ) (RDB13190) |