論文 - 詳細
RRC ID | 48815 |
---|---|
著者 | Tashiro K, Tada H, Heilker R, Shirozu M, Nakano T, Honjo T. |
タイトル | Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. |
ジャーナル | Science |
Abstract |
A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5' portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1 alpha (SDF-1 alpha) and SDF-1 beta, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned. |
巻・号 | 261(5121) |
ページ | 600-3 |
公開日 | 1993-7-30 |
DOI | 10.1126/science.8342023 |
PMID | 8342023 |
MeSH | Amino Acid Sequence Animals Base Sequence Cell Line Chemokine CXCL12 Chemokines, CXC* Cloning, Molecular / methods* Cytokines / chemistry Cytokines / genetics* Genetic Vectors Molecular Sequence Data Open Reading Frames Protein Sorting Signals / chemistry Protein Sorting Signals / genetics* Protein Sorting Signals / metabolism Receptors, Interleukin-2 / genetics Recombinant Fusion Proteins / biosynthesis Transfection |
IF | 41.846 |
引用数 | 611 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
遺伝子材料 | mouse SDF-1 alpha cDNA (RDB06778) mouse SDF-1 beta cDNA (RDB06779). |