RRC ID |
48822
|
著者 |
Muramatsu M, Sankaranand VS, Anant S, Sugai M, Kinoshita K, Davidson NO, Honjo T.
|
タイトル |
Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells.
|
ジャーナル |
J Biol Chem
|
Abstract |
We have identified a novel gene referred to as activation-induced deaminase (AID) by subtraction of cDNAs derived from switch-induced and uninduced murine B lymphoma CH12F3-2 cells, more than 80% of which switch exclusively to IgA upon stimulation. The amino acid sequence encoded by AID cDNA is homologous to that of apolipoprotein B (apoB) mRNA-editing enzyme, catalytic polypeptide 1 (APOBEC-1), a type of cytidine deaminase that constitutes a catalytic subunit for the apoB mRNA-editing complex. In vitro experiments using a glutathione S-transferase AID fusion protein revealed significant cytidine deaminase activity that is blocked by tetrahydrouridine and by zinc chelation. However, AID alone did neither demonstrate activity in C to U editing of apoB mRNA nor bind to AU-rich RNA targets. AID mRNA expression is induced in splenic B cells that were activated in vitro or by immunizations with sheep red blood cells. In situ hybridization of immunized spleen sections revealed the restricted expression of AID mRNA in developing germinal centers in which modulation of immunoglobulin gene information through somatic hypermutation and class switch recombination takes place. Taken together, these findings suggest that AID is a new member of the RNA-editing deaminase family and may play a role in genetic events in the germinal center B cell.
|
巻・号 |
274(26)
|
ページ |
18470-6
|
公開日 |
1999-6-25
|
DOI |
10.1074/jbc.274.26.18470
|
PII |
S0021-9258(19)87181-8
|
PMID |
10373455
|
MeSH |
APOBEC-1 Deaminase
Amino Acid Sequence
Animals
Apolipoproteins B
B-Lymphocytes / enzymology*
Cycloheximide / pharmacology
Cytidine Deaminase / biosynthesis*
Cytidine Deaminase / chemistry
Cytidine Deaminase / genetics
DNA, Complementary / isolation & purification
Enzyme Induction / drug effects
Gene Library
Germinal Center / cytology*
Germinal Center / enzymology
Mice
Molecular Sequence Data
Open Reading Frames
Phylogeny
Protein Synthesis Inhibitors / pharmacology
RNA Editing*
RNA, Messenger / metabolism
Sequence Alignment
Tumor Cells, Cultured
|
IF |
4.238
|
引用数 |
812
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
遺伝子材料 |
pmAID BSSK (RDB02864)
pGST-AID (RDB03001). |