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Reference Info.
Pubmed ID 18553504  
RRC ID 4916 
Author Chiang CJ, Chen PT, Chao YP. 
Title Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli. 
Journal Biotechnol. Bioeng. 
Published 2008-6-17 
Volume 101(5) 
Pages 985-95 
DOI 10.1002/bit.21976   
Attachment Sites, Microbiological / genetics*
Carotenoids / metabolism
Chromosomes / metabolism
Cloning, Molecular / methods
DNA / genetics
DNA / metabolism
DNA Nucleotidyltransferases / genetics
DNA-Directed RNA Polymerases / genetics
DNA-Directed RNA Polymerases / metabolism
Escherichia coli / genetics*
Escherichia coli / metabolism
Farnesyl-Diphosphate Farnesyltransferase / genetics
Farnesyltranstransferase / genetics
Gene Expression Regulation, Bacterial*
Gene Targeting / methods*
Genes, Regulator
Genetic Enhancement / methods
Genetic Markers
Genetic Vectors
Mutagenesis, Insertional / methods*
Oxidoreductases / genetics
Phosphoenolpyruvate Carboxykinase (ATP) / genetics
Recombination, Genetic / physiology
Replicon / genetics
Transferases / genetics
Viral Proteins / genetics
Viral Proteins / metabolism
Resource Info.
Species Prokaryotes E. coli 
Resource name
CV-25(DH5alpha), pINT-ts, pAH69, pAH121, pAH13

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