論文 - 詳細
| RRC ID | 49616 |
|---|---|
| 著者 | Imai A, Ishida M, Fukuda M, Nashida T, Shimomura H. |
| タイトル | MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor for Rab27 during granule exocytosis of rat parotid acinar cells. |
| ジャーナル | Arch Biochem Biophys |
| Abstract |
We previously reported that the small GTPase Rab27 and its effectors regulate isoproterenol (IPR)-stimulated amylase release from rat parotid acinar cells. Although activation of Rab27 by a specific guanine nucleotide exchange factor (GEF) is thought to be required for amylase release, its activation mechanism is poorly understood, because GEF for Rab27 has not been reported in parotid acinar cells. In the present study, we investigated the possible involvement of MADD/DENN/Rab3GEP, which was recently described as a Rab27-GEF in melanocytes, in amylase release from rat parotid acinar cells. Reverse transcription-PCR analyses indicated that mRNA of DENND family members, including MADD, was expressed in parotid acinar cells. MADD protein was also expressed in the cytosolic fraction of parotid acinar cells. Incubation of an antibody against the C-terminal 150 amino acids of MADD (anti-MADD-C antibody) with streptolysin O-permeabilized parotid acinar cells caused not only inhibition of IPR-induced amylase release but also reduction in the amount of GTP-Rab27. Our findings indicated that MADD functions as a GEF for Rab27 in parotid acinar cells and that its GEF activity for Rab27, i.e., GDP/GTP cycling, is required for IPR-induced amylase release. |
| 巻・号 | 536(1) |
| ページ | 31-7 |
| 公開日 | 2013-8-1 |
| DOI | 10.1016/j.abb.2013.05.002 |
| PII | S0003-9861(13)00153-7 |
| PMID | 23702376 |
| MeSH | Acinar Cells / cytology* Acinar Cells / metabolism Amylases / metabolism* Animals Bacterial Proteins / metabolism Cell Membrane / metabolism Cell Membrane Permeability / drug effects Death Domain Receptor Signaling Adaptor Proteins / analysis Death Domain Receptor Signaling Adaptor Proteins / genetics Death Domain Receptor Signaling Adaptor Proteins / metabolism* Exocytosis* Gene Expression Guanine Nucleotide Exchange Factors / analysis Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism* Guanosine Triphosphate / metabolism Humans Male Parotid Gland / cytology* RNA, Messenger / analysis RNA, Messenger / genetics Rats Rats, Wistar Streptolysins / metabolism rab GTP-Binding Proteins / metabolism* rab27 GTP-Binding Proteins |
| IF | 3.391 |
| 引用数 | 9 |
| リソース情報 | |
| 遺伝子材料 | pEGFP-C1-human DENN (RDB15288) pEGFP-C1-mouse DENND1A (RDB15289) pEGFP-C1-mouse DENND3 (RDB15290). |