RRC ID 49628
著者 Tsutsui H, Karasawa S, Okamura Y, Miyawaki A.
タイトル Improving membrane voltage measurements using FRET with new fluorescent proteins.
ジャーナル Nat Methods
Abstract We used two new coral fluorescent proteins as fluorescence resonance energy transfer (FRET) donor and acceptor to develop a voltage sensor, named Mermaid, that displays approximately 40% changes in emission ratio per 100 mV, allowing for direct visualization of electrical activities in cultured excitable cells. Notably, Mermaid has fast on-off kinetics at warm (approximately 33 degrees C) temperatures and can report voltage spikes comparable to action potentials.
巻・号 5(8)
ページ 683-5
公開日 2008-8-1
DOI 10.1038/nmeth.1235
PII nmeth.1235
PMID 18622396
MeSH Cell Membrane* Fluorescence Resonance Energy Transfer / methods* Kinetics Luminescent Proteins / analysis* Luminescent Proteins / genetics Luminescent Proteins / metabolism Membrane Potentials* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism
IF 30.822
引用数 192
リソース情報
遺伝子材料 Mermaid (RDB15262) hmKO-K/pRSETB (RDB15314) CoralHue™ monomeric Umikinoko-Green 1 (pmUkG1-S1) (RDB18306) CoralHue™ humanized monomeric Umikinoko-Green 1 (phmUkG1-S1) (RDB18307) CoralHue™ humanized monomeric Umikinoko-Green 1 (phmUkG1-MC1) (RDB18308) CoralHue™ humanized monomeric Umikinoko-Green 1 (phmUkG1-MN1) (RDB18309)