| RRC ID |
53816
|
| 著者 |
Kuroda M, Funasaki S, Saitoh T, Sasazawa Y, Nishiyama S, Umezawa K, Simizu S.
|
| タイトル |
Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.
|
| 巻・号 |
587(22)
|
| ページ |
3656-60
|
| 公開日 |
2013-11-15
|
| DOI |
10.1016/j.febslet.2013.09.017
|
| PII |
S0014-5793(13)00711-4
|
| PMID |
24076029
|
| MeSH |
Adaptor Proteins, Signal Transducing / chemistry*
Amino Acid Sequence
Binding Sites
HEK293 Cells
Humans
Membrane Proteins / chemistry*
Models, Molecular
Molecular Sequence Data
Plant Extracts / chemistry*
Protein Binding
Protein Structure, Tertiary
Vinca Alkaloids / chemistry*
|
| IF |
3.057
|
| 引用数 |
9
|
| リソース情報 |
| 遺伝子材料 |
pCI-neo/ARL6ip1(1-29)-Gluc-ARL6ip1(30-203) [V29] (RDB14554)
pCI-neo/ARL6ip1(1-37)-Gluc-ARL6ip1(38-203) [L37] (RDB14555)
pCI-neo/ARL6ip1(1-64)-Gluc-ARL6ip1(65-203) [P64] (RDB14556)
pCI-neo/ARL6ip1(1-64)-Gluc [P64*] (RDB14557)
pCI-neo/ARL6ip1(1-90)-Gluc-ARL6ip1(91-203) [R90] (RDB14558)
pCI-neo/ARL6ip1(1-99)-Gluc-ARL6ip1(100-203) [T99] (RDB14559)
pCI-neo/ARL6ip1(1-119)-Gluc-ARL6ip1(120-203) [A119] (RDB14560)
pCI-neo/ARL6ip1(1-189)-Gluc-ARL6ip1(190-203) [R189] (RDB14561)
pCI-neo/ARL6ip1(1-199)-Gluc-ARL6ip1(200-203) [E199] (RDB14562)
pCI-neo/GFP-ARL6ip1(WT) (RDB14563)
pCI-neo/GFP-ARL6ip1(Δ1-41) (RDB14564)
pCI-neo/GFP-ARL6ip1(Δ1-86) (RDB14565)
pCI-neo/GFP-ARL6ip1(Δ1-133) (RDB14566)
pCI-neo/GFP-ARL6ip1(Δ42-86) (RDB14567)
pCI-neo/GFP-ARL6ip1(42-133+KKNE) (RDB14568) |
