RRC ID 54393
著者 Hososhima S, Yuasa H, Ishizuka T, Hoque MR, Yamashita T, Yamanaka A, Sugano E, Tomita H, Yawo H.
タイトル Near-infrared (NIR) up-conversion optogenetics.
ジャーナル Sci Rep
Abstract Non-invasive remote control technologies designed to manipulate neural functions have been long-awaited for the comprehensive and quantitative understanding of neuronal network in the brain as well as for the therapy of neurological disorders. Recently, it has become possible for the neuronal activity to be optically manipulated using biological photo-reactive molecules such as channelrhodopsin (ChR)-2. However, ChR2 and its relatives are mostly reactive to visible light, which does not effectively penetrate through biological tissues. In contrast, near-infrared (NIR) light (650-1450 nm) penetrates deep into the tissues because biological systems are almost transparent to light within this so-called 'imaging window'. Here we used lanthanide nanoparticles (LNPs), composed of rare-earth elements, as luminous bodies to activate ChRs since they absorb low-energy NIR light to emit high-energy visible light (up-conversion). Here, we created a new type of optogenetic system which consists of the donor LNPs and the acceptor ChRs. The NIR laser irradiation emitted visible light from LNPs, then induced the photo-reactive responses in the near-by cells that expressed ChRs. However, there remains room for large improvements in the energy efficiency of the LNP-ChR system.
巻・号 5
ページ 16533
公開日 2015-11-10
DOI 10.1038/srep16533
PII srep16533
PMID 26552717
PMC PMC4639720
MeSH Animals Infrared Rays* Lanthanoid Series Elements Metal Nanoparticles Mice Neurons / physiology Optogenetics* Rats Spectroscopy, Near-Infrared
IF 3.998
引用数 44
リソース情報
遺伝子材料 pCMV-C1V1-Venus (RDB16793) pCMV-PsChR-Venus (RDB16794) pCaMKIIa-C1V1-Venus (RDB16795) pCaMKIIa-PsChR-Venus (RDB16796)