RRC ID 55033
著者 Osawa T, Kotani T, Kawaoka T, Hirata E, Suzuki K, Nakatogawa H, Ohsumi Y, Noda NN.
タイトル Atg2 mediates direct lipid transfer between membranes for autophagosome formation.
ジャーナル Nat Struct Mol Biol
Abstract A key event in autophagy is autophagosome formation, whereby the newly synthesized isolation membrane (IM) expands to form a complete autophagosome using endomembrane-derived lipids. Atg2 physically links the edge of the expanding IM with the endoplasmic reticulum (ER), a role that is essential for autophagosome formation. However, the molecular function of Atg2 during ER-IM contact remains unclear, as does the mechanism of lipid delivery to the IM. Here we show that the conserved amino-terminal region of Schizosaccharomyces pombe Atg2 includes a lipid-transfer-protein-like hydrophobic cavity that accommodates phospholipid acyl chains. Atg2 bridges highly curved liposomes, thereby facilitating efficient phospholipid transfer in vitro, a function that is inhibited by mutations that impair autophagosome formation in vivo. These results suggest that Atg2 acts as a lipid-transfer protein that supplies phospholipids for autophagosome formation.
巻・号 26(4)
ページ 281-288
公開日 2019-4-1
DOI 10.1038/s41594-019-0203-4
PII 10.1038/s41594-019-0203-4
PMID 30911189
MeSH Autophagosomes / metabolism* Autophagy / physiology Autophagy-Related Proteins / metabolism Carrier Proteins / metabolism Endoplasmic Reticulum / metabolism* Hydrophobic and Hydrophilic Interactions Liposomes / metabolism Phospholipids / metabolism Schizosaccharomyces / metabolism*
IF 12.109
引用数 46
リソース情報
遺伝子材料 S. pombe entry clone SpFFH31A11 (SPW052411)