| RRC ID |
55038
|
| 著者 |
Lü J, Izvolsky KI, Qian J, Cardoso WV.
|
| タイトル |
Identification of FGF10 targets in the embryonic lung epithelium during bud morphogenesis.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Genetic studies implicate Fgf10-Fgfr2 signaling as a critical regulator of bud morphogenesis in the embryo. However, little is known about the transcriptional targets of Fgf10 during this process. Here we identified global changes in gene expression in lung epithelial explants undergoing FGF10-mediated budding in the absence of other growth factors and mesenchyme. Targets were confirmed by their localization at sites where endogenous Fgf10 signaling is active in embryonic lungs and by demonstrating their induction in intact lungs in response to local application of FGF10 protein. We show that the initial stages of budding are characterized by marked up-regulation of genes associated with cell rearrangement and cell migration, inflammatory process, and lipid metabolism but not cell proliferation. We also found that some genes implicated in tumor invasion and metastatic behavior are epithelial targets of Fgf10 in the lung and other developing organs that depend on Fgf10-Fgfr2 signaling to properly form. Our approach identifies Fgf10 targets that are common to multiple biological processes and provides insights into potential mechanisms by which Fgf signaling regulates epithelial cell behavior.
|
| 巻・号 |
280(6)
|
| ページ |
4834-41
|
| 公開日 |
2005-2-11
|
| DOI |
10.1074/jbc.M410714200
|
| PII |
S0021-9258(20)76134-X
|
| PMID |
15556938
|
| MeSH |
Animals
Cell Adhesion
Cell Movement
Cell Proliferation
DNA Primers / chemistry
Embryo, Mammalian / metabolism*
Epithelium / metabolism
Fibroblast Growth Factor 10
Fibroblast Growth Factors / metabolism
Fibroblast Growth Factors / physiology*
Gene Expression Regulation, Developmental
In Situ Hybridization
Inflammation
Lung / embryology*
Mesoderm / metabolism
Mice
Oligonucleotide Array Sequence Analysis
RNA / metabolism
Receptor Protein-Tyrosine Kinases / metabolism
Receptor, Fibroblast Growth Factor, Type 2
Receptors, Fibroblast Growth Factor / metabolism
Signal Transduction
Time Factors
Transcription, Genetic
Up-Regulation
|
| IF |
4.106
|
| 引用数 |
53
|
| リソース情報 |
| 遺伝子材料 |
NIA 15K Mouse cDNA Clone
H3029F01 (MCH011721)
H3031E05 (MCH012501)
H3034H10 (MCH013778)
H3040F10 (MCH016130)
H3047A09 (MCH018809)
H3059F12 (MCH023732)
H3131D12 (MCH052484)
H3146E07 (MCH058503) |
