RRC ID 61452
著者 Tanaka Y, Yoshikaie K, Takeuchi A, Ichikawa M, Mori T, Uchino S, Sugano Y, Hakoshima T, Takagi H, Nonaka G, Tsukazaki T.
タイトル Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake.
ジャーナル Sci Adv
Abstract We have demonstrated that a bacterial membrane protein, YeeE, mediates thiosulfate uptake. Thiosulfate is used for cysteine synthesis in bacteria as an inorganic sulfur source in the global biological sulfur cycle. The crystal structure of YeeE at 2.5-Å resolution reveals an unprecedented hourglass-like architecture with thiosulfate in the positively charged outer concave side. YeeE is composed of loops and 13 helices including 9 transmembrane α helices, most of which show an intramolecular pseudo 222 symmetry. Four characteristic loops are buried toward the center of YeeE and form its central region surrounded by the nine helices. Additional electron density maps and successive molecular dynamics simulations imply that thiosulfate can remain temporally at several positions in the proposed pathway. We propose a plausible mechanism of thiosulfate uptake via three important conserved cysteine residues of the loops along the pathway.
巻・号 6(35)
ページ eaba7637
公開日 2020-8-1
DOI 10.1126/sciadv.aba7637
PII aba7637
PMID 32923628
PMC PMC7449682
IF 13.117
リソース情報
遺伝子材料 Genomic DNA of Escherichia coli K-12 JCM 20135 (JGD07547)