論文 - 詳細
| RRC ID | 67774 |
|---|---|
| 著者 | Homma Y, Fukuda M. |
| タイトル | Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Rab small GTPases regulate intracellular membrane trafficking by interacting with specific binding proteins called Rab effectors. Although Rab6 is implicated in basement membrane formation and secretory cargo trafficking, its precise regulatory mechanisms have remained largely unknown. In the present study we established five knockout cell lines for candidate Rab6 effectors and discovered that knockout of VPS52, a subunit of the GARP complex, resulted in attenuated secretion and lysosomal accumulation of secretory cargos, the same as Rab6-knockout does. We also evaluated the functional importance of the previously uncharacterized C-terminal region of VPS52 for restoring these phenotypes, as well as for the sorting of lysosomal proteins. Our findings suggest that VPS52 is an effector protein that is responsible for the Rab6-dependent secretory cargo trafficking. |
| 巻・号 | 561 |
| ページ | 151-157 |
| 公開日 | 2021-7-5 |
| DOI | 10.1016/j.bbrc.2021.05.009 |
| PII | S0006-291X(21)00778-6 |
| PMID | 34023780 |
| MeSH | Animals CRISPR-Cas Systems Cells, Cultured Dogs Gene Knockdown Techniques / methods* Golgi Apparatus Humans Intracellular Membranes Lysosomes / metabolism* Protein Transport Secretory Pathway / physiology* Vesicular Transport Proteins / antagonists & inhibitors Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* rab GTP-Binding Proteins / metabolism* |
| IF | 2.985 |
| リソース情報 | |
| 遺伝子材料 | pMRX-puro-FLAG-hVPS52 (RDB18998) pMRX-puro-FLAG-hVPS52_N670 (RDB18999) pMRX-puro-FLAG-hVPS52_N556 (RDB19000) pMRX-puro-FLAG-hVPS52_N410 (RDB19001) pMRX-bsr-ss-sfGFP (RDB19002) |