RRC ID 70110
著者 Zhang X, Sun J, Lu Y, Zhang J, Shimada K, Noda T, Zhao S, Koyano T, Matsuyama M, Zhou S, Wu J, Ikawa M, Liu M.
タイトル LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice.
ジャーナル J Cell Sci
Abstract Cilia and flagella are ancient structures that achieve controlled motor functions through the coordinated interaction based on microtubules and some attached projections. Radial spokes (RSs) facilitate the beating motion of these organelles by mediating signal transduction between dyneins and a central pair (CP) of singlet microtubules. RS complex isolation from Chlamydomonas axonemes enabled the detection of 23 radial spoke proteins (RSP1-RSP23), although the roles of some radial spoke proteins remain unknown. Recently, RSP15 has been reported to be bound to the stalk of RS2, but its homolog in mammals has not been identified. Herein, we show that Lrrc23 is an evolutionarily conserved testis-enriched gene encoding an RSP15 homolog in mice. We found that LRRC23 localizes to the RS complex within murine sperm flagella and interacts with RSPH3A and RSPH3B. The knockout of Lrrc23 resulted in male infertility due to RS disorganization and impaired motility in murine spermatozoa, whereas the ciliary beating was not significantly affected. These data indicate that LRRC23 is a key regulator that underpins the integrity of the RS complex within the flagella of mammalian spermatozoa, whereas it is dispensable in cilia. This article has an associated First Person interview with the first author of the paper.
巻・号 134(20)
公開日 2021-10-15
DOI 10.1242/jcs.259381
PII 272563
PMID 34585727
MeSH Animals Axoneme* / metabolism Cilia / metabolism Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Dyneins / metabolism Fertility / genetics Flagella / metabolism Male Mice Sperm Motility* / genetics
IF 4.573
リソース情報
遺伝子材料 pClgn-Lrrc23-FLAG (RDB19439) pClgn-Lrrc23-1D4 (RDB19440)