RRC ID 73641
著者 Chino H, Hatta T, Natsume T, Mizushima N.
タイトル Intrinsically Disordered Protein TEX264 Mediates ER-phagy.
ジャーナル Mol Cell
Abstract Certain proteins and organelles can be selectively degraded by autophagy. Typical substrates and receptors of selective autophagy have LC3-interacting regions (LIRs) that bind to autophagosomal LC3 and GABARAP family proteins. Here, we performed a differential interactome screen using wild-type LC3B and a LIR recognition-deficient mutant and identified TEX264 as a receptor for autophagic degradation of the endoplasmic reticulum (ER-phagy). TEX264 is an ER protein with a single transmembrane domain and a LIR motif. TEX264 interacts with LC3 and GABARAP family proteins more efficiently and is expressed more ubiquitously than previously known ER-phagy receptors. ER-phagy is profoundly blocked by deletion of TEX264 alone and almost completely by additional deletion of FAM134B and CCPG1. A long intrinsically disordered region of TEX264 is required for its ER-phagy receptor function to bridge the gap between the ER and autophagosomal membranes independently of its amino acid sequence. These results suggest that TEX264 is a major ER-phagy receptor.
巻・号 74(5)
ページ 909-921.e6
公開日 2019-6-6
DOI 10.1016/j.molcel.2019.03.033
PII S1097-2765(19)30257-6
PMID 31006538
MeSH Amino Acid Sequence / genetics Autophagy / genetics* Autophagy-Related Proteins / chemistry Autophagy-Related Proteins / genetics* Cell Cycle Proteins / genetics Endoplasmic Reticulum / chemistry Endoplasmic Reticulum / genetics* Endoplasmic Reticulum Stress / genetics Humans Intracellular Signaling Peptides and Proteins Intrinsically Disordered Proteins / chemistry Intrinsically Disordered Proteins / genetics* Membrane Proteins Microtubule-Associated Proteins / genetics Neoplasm Proteins / genetics Proteolysis
IF 15.584
リソース情報
遺伝子材料 pCW57-CMV-ssRFP-GFP-KDEL (RDB19767) pMRX-INU-FLAG-RTN3L (RDB19781)