RRC ID 11097
著者 Yamamoto-Hino M, Kanie Y, Awano W, Aoki-Kinoshita KF, Yano H, Nishihara S, Okano H, Ueda R, Kanie O, Goto S.
タイトル Identification of genes required for neural-specific glycosylation using functional genomics.
ジャーナル PLoS Genet
Abstract Glycosylation plays crucial regulatory roles in various biological processes such as development, immunity, and neural functions. For example, α1,3-fucosylation, the addition of a fucose moiety abundant in Drosophila neural cells, is essential for neural development, function, and behavior. However, it remains largely unknown how neural-specific α1,3-fucosylation is regulated. In the present study, we searched for genes involved in the glycosylation of a neural-specific protein using a Drosophila RNAi library. We obtained 109 genes affecting glycosylation that clustered into nine functional groups. Among them, members of the RNA regulation group were enriched by a secondary screen that identified genes specifically regulating α1,3-fucosylation. Further analyses revealed that an RNA-binding protein, second mitotic wave missing (Swm), upregulates expression of the neural-specific glycosyltransferase FucTA and facilitates its mRNA export from the nucleus. This first large-scale genetic screen for glycosylation-related genes has revealed novel regulation of fucTA mRNA in neural cells.
巻・号 6(12)
ページ e1001254
公開日 2010-12-23
DOI 10.1371/journal.pgen.1001254
PMID 21203496
PMC PMC3009669
MeSH Animals Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Genomics* Glycosylation Nervous System / metabolism Organ Specificity
IF 5.175
引用数 21
WOS 分野 GENETICS & HEREDITY
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