| RRC ID |
11319
|
| 著者 |
Suzuki MG, Imanishi S, Dohmae N, Asanuma M, Matsumoto S.
|
| タイトル |
Identification of a male-specific RNA binding protein that regulates sex-specific splicing of Bmdsx by increasing RNA binding activity of BmPSI.
|
| ジャーナル |
Mol Cell Biol
|
| Abstract |
Bmdsx is a sex-determining gene in the silkworm and is alternatively spliced in males and females. CE1 is a splicing silencer element responsible for the sex-specific splicing of Bmdsx. To identify sex-specific factors implicated in the sex-specific splicing of Bmdsx, we performed RNA affinity chromatography using CE1 RNA as a ligand. We have identified BmIMP, a Bombyx homolog of IGF-II mRNA binding protein (IMP), as a male-specific factor that specifically binds to CE1. The gene encoding BmIMP is localized on the Z chromosome and is male-specifically expressed in various tissues. Antisense inhibition of BmIMP expression increased female-specific splicing of Bmdsx pre-mRNA. Coimmunoprecipitation and glutathione S-transferase (GST) pulldown analyses demonstrated that BmIMP physically interacts with BmPSI, which has been identified as a factor implicated in the sex-specific splicing of Bmdsx, through the KH domains of BmIMP. The functional consequence of this interaction was examined using RNA mobility shift analysis. BmIMP increased BmPSI-CE1 RNA binding activity by decreasing the rate of BmPSI dissociation from CE1 RNA. Truncation analysis of BmIMP suggested that the KH domains are responsible for enhancing BmPSI-CE1 RNA binding activity. These results suggest that BmIMP may enhance the male-specific splicing of Bmdsx pre-mRNA by increasing RNA binding activity of BmPSI.
|
| 巻・号 |
30(24)
|
| ページ |
5776-86
|
| 公開日 |
2010-12-1
|
| DOI |
10.1128/MCB.00444-10
|
| PII |
MCB.00444-10
|
| PMID |
20956562
|
| PMC |
PMC3004276
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Female
Insect Proteins / genetics
Insect Proteins / metabolism*
Male
Molecular Sequence Data
RNA Interference
RNA Splicing*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism*
Sequence Alignment
Sex Characteristics*
Tissue Distribution
|
| IF |
3.611
|
| 引用数 |
36
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
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| リソース情報 |
| カイコ |
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