RRC ID 11409
Author Jun J, Fiume E, Roeder AH, Meng L, Sharma VK, Osmont KS, Baker C, Ha CM, Meyerowitz EM, Feldman LJ, Fletcher JC.
Title Comprehensive analysis of CLE polypeptide signaling gene expression and overexpression activity in Arabidopsis.
Journal Plant Physiol.
Abstract Intercellular signaling is essential for the coordination of growth and development in higher plants. Although hundreds of putative receptors have been identified in Arabidopsis (Arabidopsis thaliana), only a few families of extracellular signaling molecules have been discovered, and their biological roles are largely unknown. To expand our insight into the developmental processes potentially regulated by ligand-mediated signal transduction pathways, we undertook a systematic expression analysis of the members of the Arabidopsis CLAVATA3/ESR-RELATED (CLE) small signaling polypeptide family. Using reporter constructs, we show that the CLE genes have distinct and specific patterns of promoter activity. We find that each Arabidopsis tissue expresses at least one CLE gene, indicating that CLE-mediated signaling pathways are likely to play roles in many biological processes during the plant life cycle. Some CLE genes that are closely related in sequence have dissimilar expression profiles, yet in many tissues multiple CLE genes have overlapping patterns of promoter-driven reporter activity. This observation, plus the general absence of detectable morphological phenotypes in cle null mutants, suggest that a high degree of functional redundancy exists among CLE gene family members. Our work establishes a community resource of CLE-related biological materials and provides a platform for understanding and ultimately manipulating many different plant signaling systems.
Volume 154(4)
Pages 1721-36
Published 2010-12
DOI 10.1104/pp.110.163683
PII pp.110.163683
PMID 20884811
PMC PMC2996011
MeSH Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Flowers / metabolism Genes, Plant Plant Roots / metabolism Promoter Regions, Genetic Signal Transduction*
IF 5.949
Times Cited 61
Arabidopsis / Cultured plant cells, genes pst12990 pst17487 pst10796