RRC ID 12007
著者 Matsumoto T, Tanaka T, Sakai H, Amano N, Kanamori H, Kurita K, Kikuta A, Kamiya K, Yamamoto M, Ikawa H, Fujii N, Hori K, Itoh T, Sato K.
タイトル Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.
ジャーナル Plant Physiol
Abstract Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.
巻・号 156(1)
ページ 20-8
公開日 2011-5-1
DOI 10.1104/pp.110.171579
PII pp.110.171579
PMID 21415278
PMC PMC3091036
MeSH Base Sequence DNA, Complementary / chemistry DNA, Complementary / genetics* Gene Library Genome, Plant / genetics* Hordeum / genetics* Molecular Sequence Data RNA, Messenger / genetics RNA, Plant / genetics Sequence Analysis, DNA
IF 6.902
引用数 148
WOS 分野 PLANT SCIENCES
リソース情報
オオムギ FLcDNAs