RRC ID 1299
著者 Mori K, Muto Y, Kokuzawa J, Yoshioka T, Yoshimura S, Iwama T, Okano Y, Sakai N.
タイトル Neuronal protein NP25 interacts with F-actin.
ジャーナル Neurosci Res
Abstract Neuronal protein NP25 is a neuron-specific protein present in highly differentiated neural cells, but its functional properties have not been well characterized. NP25 shows high amino acid sequence homology with the smooth muscle cell cytoskeleton-associated proteins, SM22, mp20, and calponin. To gain an insight into the biological functions of NP25, we first examined its subcellular localization in the human neuroblastoma cell line, SK-N-SH. NP25 diffusely distributed in the cytoplasm and fiber-like staining was also observed. It showed that NP25 co-localized with F-actin on stress fibers. A co-sedimentation assay demonstrated that NP25 bound to filamentous actin. Further investigations using fluorescence resonance energy transfer (FRET) technique revealed intracellular binding of NP25 and actin. The significance of the interaction between NP25 and F-actin is discussed.
巻・号 48(4)
ページ 439-46
公開日 2004-4-1
DOI 10.1016/j.neures.2003.12.012
PII S0168010203003481
PMID 15041197
MeSH Actins / metabolism* Animals Blotting, Western Cell Line, Tumor Cytoskeleton / metabolism Immunohistochemistry Microscopy, Fluorescence Nerve Tissue Proteins / metabolism* Neuroblastoma / metabolism
IF 2.645
引用数 24
WOS 分野 NEUROSCIENCES
リソース情報
ヒト・動物細胞 SK-N-SH(RCB0426)