RRC ID 15576
著者 Agrawal P, Habib F, Yelagandula R, Shashidhara LS.
タイトル Genome-level identification of targets of Hox protein Ultrabithorax in Drosophila: novel mechanisms for target selection.
ジャーナル Sci Rep
Abstract Hox proteins are transcription factors and key regulators of segmental identity along the anterior posterior axis across all bilaterian animals. Despite decades of research, the mechanisms by which Hox proteins select and regulate their targets remain elusive. We have carried out whole-genome ChIP-chip experiments to identify direct targets of Hox protein Ultrabithorax (Ubx) during haltere development in Drosophila. Direct targets identified include upstream regulators or cofactors of Ubx. Homothorax, a cofactor of Ubx during embryonic development, is one such target and is required for normal specification of haltere. Although Ubx bound sequences are conserved amongst various insect genomes, no consensus Ubx-specific motif was detected. Surprisingly, binding motifs for certain transcription factors that function either upstream or downstream to Ubx are enriched in these sequences suggesting complex regulatory loops governing Ubx function. Our data supports the hypothesis that specificity during Hox target selection is achieved by associating with other transcription factors.
巻・号 1
ページ 205
公開日 2011-12-22
DOI 10.1038/srep00205
PII srep00205
PMID 22355720
PMC PMC3244697
MeSH Amino Acid Motifs Animals Antibodies, Monoclonal / metabolism Chromatin Immunoprecipitation Down-Regulation Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology Drosophila melanogaster / genetics* Gene Expression Profiling Gene Expression Regulation, Developmental* Genome, Insect Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* RNA Interference Transcription Factors / genetics Transcription Factors / metabolism*
IF 3.998
引用数 20
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 17117R-2