RRC ID 1691
Author Gil R, Silva FJ, Peretó J, Moya A.
Title Determination of the core of a minimal bacterial gene set.
Journal Microbiol. Mol. Biol. Rev.
Abstract The availability of a large number of complete genome sequences raises the question of how many genes are essential for cellular life. Trying to reconstruct the core of the protein-coding gene set for a hypothetical minimal bacterial cell, we have performed a computational comparative analysis of eight bacterial genomes. Six of the analyzed genomes are very small due to a dramatic genome size reduction process, while the other two, corresponding to free-living relatives, are larger. The available data from several systematic experimental approaches to define all the essential genes in some completely sequenced bacterial genomes were also considered, and a reconstruction of a minimal metabolic machinery necessary to sustain life was carried out. The proposed minimal genome contains 206 protein-coding genes with all the genetic information necessary for self-maintenance and reproduction in the presence of a full complement of essential nutrients and in the absence of environmental stress. The main features of such a minimal gene set, as well as the metabolic functions that must be present in the hypothetical minimal cell, are discussed.
Volume 68(3)
Pages 518-37, table of contents
Published 2004-9
DOI 10.1128/MMBR.68.3.518-537.2004
PII 68/3/518
PMID 15353568
PMC PMC515251
MeSH Bacteria / genetics Bacteria / growth & development* Bacteria / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism* Computational Biology Genes, Bacterial* Genome, Bacterial*
IF 15.255
Times Cited 284