| RRC ID |
1691
|
| 著者 |
Gil R, Silva FJ, Peretó J, Moya A.
|
| タイトル |
Determination of the core of a minimal bacterial gene set.
|
| ジャーナル |
Microbiol Mol Biol Rev
|
| Abstract |
The availability of a large number of complete genome sequences raises the question of how many genes are essential for cellular life. Trying to reconstruct the core of the protein-coding gene set for a hypothetical minimal bacterial cell, we have performed a computational comparative analysis of eight bacterial genomes. Six of the analyzed genomes are very small due to a dramatic genome size reduction process, while the other two, corresponding to free-living relatives, are larger. The available data from several systematic experimental approaches to define all the essential genes in some completely sequenced bacterial genomes were also considered, and a reconstruction of a minimal metabolic machinery necessary to sustain life was carried out. The proposed minimal genome contains 206 protein-coding genes with all the genetic information necessary for self-maintenance and reproduction in the presence of a full complement of essential nutrients and in the absence of environmental stress. The main features of such a minimal gene set, as well as the metabolic functions that must be present in the hypothetical minimal cell, are discussed.
|
| 巻・号 |
68(3)
|
| ページ |
518-37, table of contents
|
| 公開日 |
2004-9-1
|
| DOI |
10.1128/MMBR.68.3.518-537.2004
|
| PII |
68/3/518
|
| PMID |
15353568
|
| PMC |
PMC515251
|
| MeSH |
Bacteria / genetics
Bacteria / growth & development*
Bacteria / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Computational Biology
Genes, Bacterial*
Genome, Bacterial*
|
| IF |
12.568
|
| 引用数 |
356
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 情報 |
E.coli |
