| RRC ID |
2064
|
| 著者 |
Ghosh M, Koike N, Yanagimoto G, Tsunoda S, Kaul S, Hirano T, Emura F, Kashiwagi H, Kawamoto T, Ohkohchi N, Saijo K, Ohno T, Miwa M, Todoroki T.
|
| タイトル |
Establishment and characterization of unique human gallbladder cancer cell lines.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
Gallbladder cancer has a dismal prognosis. Understanding the disease at the biological, genetic, molecular, cellular, and clinical level is essential for effective diagnostics and therapeutics. However, the currently established gallbladder cell lines are insufficient for better understanding and further research. The aim of our present study was to establish and characterize human gallbladder cancer cell lines. We established 5 cell lines from resected specimens of gallbladder cancers. These cell lines revealed typical tumor histopathological characteristics. We examined growth characteristics and the colony-forming ability of established cell lines in terms of their cell cycle parameters, expression of tumor markers (carcinoembryonic antigen; CEA, carbohydrated antigen 19-9; CA19-9, MUC-1 and c-kit) and the oncogene c-erbB2 by flow cytometer. Comparative genomic hybridization (CGH) analysis with specific gene probes was performed to detect changes in the gene copy numbers. Human origin of cell lines was confirmed by chromosomal analysis. Cells maintained differentiation characteristics of the original tumors. The doubling time of different cell lines varied from 30 to 96 h. All 5 cell lines formed colonies in the colony forming assays and expressed CEA, CA19-9, MUC-1 and the oncogene c-erbB2 and showed chromosomal aneuploidy. CGH analysis demonstrated gain of chromosomal region bearing SRC, RAB1, and PAP in all cell lines and hTERT in 4 cell lines. These newly established cell lines might serve as a useful model for studying the molecular pathogenesis of gallbladder cancer. Furthermore, they may serve as a model for testing new therapeutics against gallbladder cancer. These chromosomal aberrations and imbalances provide a starting point for molecular analyses of genomic regions and genes in gallbladder carcinogenesis.
|
| 巻・号 |
24(5)
|
| ページ |
1189-96
|
| 公開日 |
2004-5-1
|
| PMID |
15067341
|
| MeSH |
Animals
Biomarkers, Tumor / metabolism*
CA-19-9 Antigen / metabolism
Carcinoembryonic Antigen / metabolism
Cell Cycle
Cell Division
Chromosome Aberrations
Colony-Forming Units Assay
Gallbladder Neoplasms / metabolism
Gallbladder Neoplasms / pathology*
Gene Amplification
Gene Dosage
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Mucin-1 / metabolism
Nucleic Acid Hybridization
Proto-Oncogene Proteins c-kit / metabolism
Receptor, ErbB-2 / metabolism
Tumor Cells, Cultured*
|
| IF |
3.899
|
| 引用数 |
18
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
TGBC1TKB(RCB1129)
TGBC2TKB(RCB1130)
TGBC14TKB(RCB1186)
TGBC24TKB(RCB1196)
TGBC44TKB(RCB1259) |
