論文 - 詳細
| RRC ID | 2189 |
|---|---|
| 著者 | Sone T, Nagamori E, Ikeuchi T, Mizukami A, Takakura Y, Kajiyama S, Fukusaki E, Harashima S, Kobayashi A, Fukui K. |
| タイトル | A novel gene delivery system in plants with calcium alginate micro-beads. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
We have produced micrometer-sized calcium alginate beads referred to as "bio-beads" that encapsulate plasmid DNA molecules carrying a reporter gene. In order to evaluate the efficiency of the bio-beads in mediating genetic transfection, protoplasts isolated from cultured tobacco cells (BY-2) were transfected with bio-beads containing a plasmid that carries the modified green fluorescent protein gene CaMV35S-sGFP. With the bio-beads treatment, approximately ten-fold higher GFP expression was observed after 24 h incubation compared to that with the conventional method using a naked plasmid solution. Transfection was up to 0.22% efficient. These results indicate that bio-beads have a possibility for efficient transformation in plants. |
| 巻・号 | 94(1) |
| ページ | 87-91 |
| 公開日 | 2002-1-1 |
| DOI | 10.1263/jbb.94.87 |
| PII | S1389-1723(02)80123-2 |
| PMID | 16233276 |
| IF | 2.366 |
| 引用数 | 37 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| リソース情報 | |
| 酵母 | pUC Plasmid |