RRC ID 3220
Author Kusumoto M, Suzuki R, Nishiya Y, Okitsu T, Oka M.
Title Host-dependent activation of IS1203v excision in Shiga toxin-producing Escherichia coli.
Journal J. Biosci. Bioeng.
Abstract IS1203v is an insertion sequence (IS) which is identical to the most abundant IS elements in the genome of Escherichia coli O157:H7. However, there is no sequence homologous to IS1203v in the genome of E. coli K-12. We constructed a system to analyze the excision frequency of IS1203v, and demonstrated that the frequency in E. coli O157:H7 was approximately 10(5) times higher than that in E. coli K-12. We also investigated the excision frequencies of IS1203v in various E. coli isolates, and showed that the excision frequencies of IS1203v-possessing strains were approximately 10(3) times higher than those of IS1203v-nonpossessing strains. The results suggest that the IS1203v-possessing strains use a common system to enhance IS1203v excision.
Volume 97(6)
Pages 406-11
Published 2004
DOI 10.1016/S1389-1723(04)70227-3
PII S1389-1723(04)70227-3
PMID 16233651
IF 2.015
Times Cited 3
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Prokaryotes E. coli ME7986(MG1655)