RRC ID 32715
Author Yumura S, Hashima S, Muranaka S.
Title Myosin II does not contribute to wound repair in Dictyostelium cells.
Journal Biol Open
Abstract Cells are always subjected to mechanical stresses, resulting in wounds of the cell membrane, but cells are able to repair and reseal their wounded membrane. Previous reports have shown that actin and myosin II accumulate around the wound and that the constriction of this purse-string closes the membrane pore. Here, we developed a microsurgical wound assay to assess wound repair in Dictyostelium cells. Fluorescent dye that had been incorporated into the cells leaked out for only 2-3 sec after wounding, and a GFP-derived, fluorescent Ca(2+) sensor showed that intracellular Ca(2+) transiently increased immediately after wounding. In the absence of external Ca(2+), the cell failed to repair itself. During the repair process, actin accumulated at the wounded sites but myosin II did not. The wounds were repaired even in myosin II null cells to a comparable degree as the wild-type cells, suggesting that myosin II does not contribute to wound repair. Thus, the actomyosin purse-string constriction model is not a common mechanism for wound repair in eukaryotic cells, and this discrepancy may arise from the difference in cell size.
Volume 3(10)
Pages 966-73
Published 2014-9-19
DOI 10.1242/bio.20149712
PII bio.20149712
PMID 25238760
PMC PMC4197445
IF 2.217
Times Cited 2
WOS Category BIOLOGY
Resource
Cellular slime molds S00009