RRC ID 34100
著者 Mingma R, Duangmal K, Trakulnaleamsai S, Thamchaipenet A, Matsumoto A, Takahashi Y.
タイトル Sphaerisporangium rufum sp. nov., an endophytic actinomycete from roots of Oryza sativa L.
ジャーナル Int J Syst Evol Microbiol
Abstract An endophytic actinomycete, strain R10-82(T), isolated from surface-sterilized roots of rice (Oryza sativa L.) was studied using a polyphasic approach. Strain R10-82(T) produced branching substrate mycelia and developed spherical spore vesicles on aerial hyphae containing non-motile spores. The major cellular fatty acids were iso-C16 : 0, iso-C14 : 0 and 10-methyl C17 : 0. The predominant menaquinones were MK-9, MK-9(H2), MK-9(H4) and MK-9(H6). Rhamnose, ribose, madurose, mannose and glucose were detected in whole-cell hydrolysates. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannosides, hydroxylphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. These morphological and chemotaxonomic data were similar to those of the genus Sphaerisporangium. Analysis of the 16S rRNA gene sequence revealed that strain R10-82(T) was related most closely to Sphaerisporangium cinnabarinum JCM 3291(T) (98.3 % similarity). The DNA G+C content of strain R10-82(T) was 74 mol%. DNA-DNA relatedness data in combination with differences in the biochemical and physiological properties suggested that strain R10-82(T) should be classified as representing a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rufum is proposed. The type strain is R10-82(T) ( = BCC 51287(T) = NBRC 109079(T)). An emended description of the genus Sphaerisporangium is also provided.
巻・号 64(Pt 4)
ページ 1077-1082
公開日 2014-4-1
DOI 10.1099/ijs.0.057885-0
PMID 24363300
MeSH Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / isolation & purification Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Molecular Sequence Data Nucleic Acid Hybridization Oryza / microbiology* Phospholipids / chemistry Phylogeny* Plant Roots / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Thailand Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 15
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 3291