RRC ID 3442
Author Lanjuin A, Claggett J, Shibuya M, Hunter CP, Sengupta P.
Title Regulation of neuronal lineage decisions by the HES-related bHLH protein REF-1.
Journal Dev. Biol.
Abstract Members of the HES subfamily of bHLH proteins play crucial roles in neural patterning via repression of neurogenesis. In C. elegans, loss-of-function mutations in ref-1, a distant nematode-specific member of this subfamily, were previously shown to cause ectopic neurogenesis from postembryonic lineages. However, while the vast majority of the nervous system in C. elegans is generated embryonically, the role of REF-1 in regulating these neural lineage decisions is unknown. Here, we show that mutations in ref-1 result in the generation of multiple ectopic neuron types derived from an embryonic neuroblast. In wild-type animals, neurons derived from this sublineage are present in a left/right symmetrical manner. However, in ref-1 mutants, while the ectopically generated neurons exhibit gene expression profiles characteristic of neurons on the left, they are present only on the right side. REF-1 functions in a Notch-independent manner to regulate this ectopic lineage decision. We also demonstrate that loss of REF-1 function results in defective differentiation of an embryonically generated serotonergic neuron type. These results indicate that REF-1 functions in both Notch-dependent and independent pathways to regulate multiple developmental decisions in different neuronal sublineages.
Volume 290(1)
Pages 139-51
Published 2006-2-1
DOI 10.1016/j.ydbio.2005.11.018
PII S0012-1606(05)00811-0
PMID 16376329
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism* Body Patterning* Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Differentiation Cell Lineage* DNA-Binding Proteins / metabolism Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Mutation Neurons / cytology* Neurons / metabolism Receptors, Notch / metabolism Transcription Factors / metabolism
IF 3.262
Times Cited 5
C.elegans tm458 tm284