RRC ID 36160
著者 Yalgin C, Ebrahimi S, Delandre C, Yoong LF, Akimoto S, Tran H, Amikura R, Spokony R, Torben-Nielsen B, White KP, Moore AW.
タイトル Centrosomin represses dendrite branching by orienting microtubule nucleation.
ジャーナル Nat Neurosci
Abstract Neuronal dendrite branching is fundamental for building nervous systems. Branch formation is genetically encoded by transcriptional programs to create dendrite arbor morphological diversity for complex neuronal functions. In Drosophila sensory neurons, the transcription factor Abrupt represses branching via an unknown effector pathway. Targeted screening for branching-control effectors identified Centrosomin, the primary centrosome-associated protein for mitotic spindle maturation. Centrosomin repressed dendrite branch formation and was used by Abrupt to simplify arbor branching. Live imaging revealed that Centrosomin localized to the Golgi cis face and that it recruited microtubule nucleation to Golgi outposts for net retrograde microtubule polymerization away from nascent dendrite branches. Removal of Centrosomin enabled the engagement of wee Augmin activity to promote anterograde microtubule growth into the nascent branches, leading to increased branching. The findings reveal that polarized targeting of Centrosomin to Golgi outposts during elaboration of the dendrite arbor creates a local system for guiding microtubule polymerization.
巻・号 18(10)
ページ 1437-45
公開日 2015-10-1
DOI 10.1038/nn.4099
PII nn.4099
PMID 26322925
MeSH Animals Animals, Genetically Modified Cell Polarity Chromatin Immunoprecipitation Dendrites / metabolism* Drosophila Proteins / metabolism* Drosophila melanogaster / metabolism* Homeodomain Proteins / metabolism* Microtubules / metabolism* Neurogenesis / physiology* Polymerase Chain Reaction Sensory Receptor Cells / metabolism
IF 20.071
引用数 52
WOS 分野 NEUROSCIENCES
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